Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of C.elegans and M.musculus in the presence and absence of D-Glucosamine (GlcN)

ABSTRACT: RNA-seq of C.elegans and M.musculus in the presence and absence of D-Glucosamine (GlcN)

PROVIDER: PRJNA237825 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR1166890.fastq.gz	Fastqsanger.gz
	SRR1166900.fastq.gz	Fastqsanger.gz
	SRR1166891.fastq.gz	Fastqsanger.gz
	SRR1166901.fastq.gz	Fastqsanger.gz
	SRR1166892.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

RNA-seq of C.elegans and M.musculus in the presence and absence of D-Glucosamine (GlcN)

Project description:D-Glucosamine (2-amino-2-deoxy-D-glucose, C.A.S.# 3416-24-8) (GlcN) is a freely available and commonly used dietary supplement possibly promoting cartilage health in humans which also acts as an inhibitor of glycolysis. We here find that GlcN extends C. elegans lifespan by impairing glucose metabolism to activate AMP-activated protein kinase (AMPK/AAK2) leading to increased mitochondrial biogenesis. Consistent with the concept of mitohormesis, this promotes increased formation of mitochondrial reactive oxygen species (ROS) and p38/PMK-1-mediated stress signaling culminating in increased expression of the nematodal amino acid-transporter 1 (aat-1) gene. Ameliorating mitochondrial ROS formation as well as impairment of aat-1-expression abolishes GlcN-mediated lifespan extension in a NRF2/SKN-1-dependent fashion. Notably and unlike other calorie restriction mimetics (CRM) like 2-deoxy-D-glucose (2DG, DOG), GlcN extends lifespan of aging C57BL/6 mice (log-rank: p=0.002; cox regression: p=0.01) similarly paralleled by an induction of mitochondrial biogenesis, increased expression of several murine amino acid transporters, as well as increased amino-acid catabolism. Taken together, GlcN mimics a ketogenic diet to extend healthspan in evolutionary distinct species. 24 samples: 12 mRNA profiles of C.elegans: 6 without GlcN and 6 with GlcN supplementaion; 12 mRNA profiles of M.musculus: 6 without GlcN and 6 with GlcN supplementaion

2014-03-07 | E-GEOD-54853 | biostudies-arrayexpress

RNA-seq of C.elegans and M.musculus in the presence and absence of D-Glucosamine (GlcN)

2014-03-07 | GSE54853 | GEO

Yeast glucosamine treatment

Project description:We did transcription profiling on the effect of glucosamine in Saccharomyces cerevisiae using Research Genetics strains BY4741 (wild type) and 5251 (fks1). Yeast cells exposed to glucosamine in YPD growth medium show a significant increase in chitin content in the lateral cell wall. Likewise, cell wall stress caused by a gene deletion e.g., fks1 also results in elevated chitin. By comparing our data for fks1 with those from the literature we confirmed a strong induction of genes responsive to cell wall integrity, environmental stress as well as genes involved in cell signaling. Wild type cells treated with 15 mM glucosamine for 2 hours did not show any significant change in their transcription profile although the chitin level doubled during this time. For cells continuously exposed to glucosamine (steady state) there was a moderate transcription response, 105 genes showed a two-fold or higher change. The largest groups of up-regulated genes were those involved in mating, sporulation and cell cycle arrest. The largest group of down-regulated genes pertained to mitochondrial respiration. There was little overlap between those genes that have altered transcription from glucosamine treatment and those responsive to the fks1 mutation. Keywords = saccharomyces chitin glucosamine Keywords: parallel sample

2003-05-30 | GSE441 | GEO

CD4+ T cells in presence or absence of Ikaros

Project description:CD4+ T cells in presence or absence of Ikaros

| PRJNA662885 | ENA

HOXB13 expression in T47D cells in presence/absence of estradiol

Project description:HOXB13 expression in T47D cells in presence/absence of estradiol

| PRJNA1107570 | ENA

The analysis of GSDME-NT binding proteins in the presence or absence of methionine

Project description:Our study identify that methionine inhibits N-terminus of GSDME (GSDME-NT) -mediated pyroptosis by dampening its endocytotic degradation. Thus，the binding proteins of GSDME-NT are detected in the presence or absence of Methionine for futher exploration。

2025-01-19 | PXD059954 |

Glucosamine profiling of lymphocyte derived cell lines

Project description:Define regulated genes upon 24 hours of glucosamine treatment in KMH2 and Ramos

2014-08-18 | GSE49185 | GEO

C.elegans iron homoeostasis

Project description:C.elegans iron homoeostasis

| PRJEB89976 | ENA

RNA-seq of human colorectal cancer cells in presence and absence of ZEB1

Project description:RNA-seq of human colorectal cancer cells in presence and absence of ZEB1

| PRJNA508727 | ENA

RNA-SEQ analysis of CD4+ T cells in presence or absence of Ikaros.

Project description:RNA-SEQ analysis of CD4+ T cells in presence or absence of Ikaros.