Rattus norvegicus
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ABSTRACT:
PROVIDER: PRJNA238504 | ENA |
REPOSITORIES: ENA
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Project description:Profiling of miRNA expressions comparing standard fracture healing models with nonunion models in rats 12w, male, Sprague–Dawley rats were used in this study. Animals were randomized to receive either a surgical treatment that has been shown to produce a nonunion or to a standard stabilized closed femoral shaft fracture that is known to successfully heal. The details of these procedures have been previously described. Briefly, to produce standard healing models, a 1.2-mm diameter K-wire was inserted retrograde into the right femoral intramedullary canal and a closed transverse femoral shaft fracture was produced using a three-point bending apparatus with a drop weight . To produce the nonunion, the fractured site was additionally exposed, and the periosteum was cauterized circumferentially for a distance of 2 mm on each side of the fracture . Five animals from each group were euthanized on post-fracture day 14 for microarray analysis.
2015-09-03 | E-GEOD-55088 | biostudies-arrayexpress
Project description:Profiling of miRNA expressions comparing standard fracture healing models with nonunion models in rats
2015-09-03 | GSE55088 | GEO
Project description:Bone regeneration is a highly efficient process allowing scarless healing after injury. Yet, musculoskeletal traumatic injury, when fracture is combined with adjacent muscle injury, alters bone healing leading to fibrous nonunion. The periosteum, the outer layer of bones, is a critical source of skeletal stem/progenitor cells (SSPCs), as well as immune, endothelial and neural cells during bone repair. We generated single nuclei datasets from the injured perioteum and hematoma at day 1-post fracture and from the hematoma/callus at day 5 post-musculoskeletal traumatic injury. These datasets were analyzed in combination with datasets from GSE234451.
2024-10-22 | GSE268276 | GEO
Project description:After injury, bone tissue initiates a reparative response that will recover its structure and function, failure to initiate or delay this response results in fracture nonunion. The molecular mechanisms underlying fracture nonunion appearance are not yet stablished. We proposed that the signaling pathways derived from hypoxia and mediated by reactive oxygen species (ROS) homeostasis control Bmp2 expression and fracture healing initiation, having potential to induce fracture nonunion when excessive ROS develops into oxidative stress. Here, we silenced Apex1, the final transducer of ROS signaling and responsible of the activation of key transcription factors by oxidoreduction of cysteines, evaluating its role during endochondral ossification and fracture repair. Silencing Apex1 in limb bud mesenchyme results in transient metaphyseal dysplasia derived of impaired chondrocyte differentiation. During bone regeneration, silencing Apex1 generates a fracture nonunion phenotype characterized by delayed fracture healing initiation with impaired periosteal response and reduced chondrocyte and osteoblasts differentiation. In addition, compromised chondrocyte differentiation hampers fracture healing progression and callus vascularization. Our data supports a molecular mechanism of fracture healing initiation dependent of hypoxia derived ROS signaling in mesenchymal progenitors.
2025-11-24 | GSE278433 | GEO
Project description:This is a study of femoral fracture healing in female rats 16 weeks old at fracture to compare intramedullary nailing, screw and plate fixation, and sham surgery. The sham surgery group received a surgical exposure of the femur, but no fracture, no plate, and no nail. Samples were collected at 1 day, 3 days, 1 week, 2 weeks, 4 weeks, and 6 weeks after surgery. Each sample is a pool of RNA from three rats from the same surgery group at the same time point after fracture. The middle third of the femur was collected with the cortical bone, fracture callus, and marrow elements. Mid-diaphyseal, simple, transverse fractures were induced by a Gigli saw. The no fracture sample was a time 0 control collected on the day of surgery from intact rats.
2007-11-03 | E-GEOD-1685 | biostudies-arrayexpress
Project description:Age-related delays in fracture healing are prevalent and contribute to morbidity and mortality in elderly populations. Clinical and preclinical studies demonstrate that aging is associated with slower and less complete fracture repair characterized by delayed cartilage and bone formation, impaired matrix resorption, and an increased risk of delayed union or nonunion. Matrix Assisted Laser Desorption Ionization Mass Spectrometry Imaging (MALDI MSI) enables spatially resolved, in situ molecular analysis of proteins directly from murine fracture tissue sections. We applied collagenase type III (MMP 13) mediated proteolytic digestion to formalin fixed, paraffin embedded (FFPE) tibia fracture callus sections harvested 10 days post tibial fracture from young (3 month old) and aged (18 month old) mice to perform spatially resolved proteomic profiling. MALDI MSI revealed pronounced age dependent differences in extracellular matrix protein composition and remodeling within the fracture callus. We identified up regulation of canonical bone and matrix proteins, including Col1a1 and Col1a2 specifically in the young fracture callus demonstrating advancement into harden callus formation. Conversely, Col2a1 and other soft callus proteins were only seen in the aged callus tissues. Further, protein indicators of tissue state, such as fibronectin (up regulated) and calreticulin (down regulated) were selectively regulated aged tissues, demonstrating a failure for aged tissues to fully progress into harden calluses. Spatial proteomic patterns demonstrated a marked delay in progression from cartilaginous to osseous callus in aged mice, consistent with impaired matrix remodeling during fracture repair. Together, these findings establish MALDI MSI based spatial proteomics as a powerful approach to elucidate age related alterations in fracture healing and to identify molecular regulators of impaired skeletal regeneration.
2025-12-22 | MSV000100309 | MassIVE
Project description:This is a study of femoral fracture healing in female rats 16 weeks old at fracture to compare intramedullary nailing, screw and plate fixation, and sham surgery. The sham surgery group received a surgical exposure of the femur, but no fracture, no plate, and no nail. Samples were collected at 1 day, 3 days, 1 week, 2 weeks, 4 weeks, and 6 weeks after surgery. Each sample is a pool of RNA from three rats from the same surgery group at the same time point after fracture. The middle third of the femur was collected with the cortical bone, fracture callus, and marrow elements. Mid-diaphyseal, simple, transverse fractures were induced by a Gigli saw. The no fracture sample was a time 0 control collected on the day of surgery from intact rats. Keywords = rat Keywords = fracture Keywords = plate Keywords = nail Keywords = time Keywords = femur Keywords: time-course
2005-01-01 | GSE1685 | GEO
Project description:A study of rat femoral fracture healing in young (6 weeks old at fracture), adult (26 weeks old at fracture), and old (52 weeks old at fracture) rats. Samples were collected at time of surgery (intact controls) and at 3 days, 1 week, 2 weeks, 4 weeks, and 6 weeks after fracture. Samples were the mid third of the femoral length including the external callus, cortical bone and marrow elements. Fracture was stabilized with an intramedullary rod prior to fracture with a Bonnarens and Einhorn device.
2007-11-03 | E-GEOD-1371 | biostudies-arrayexpress
Project description:We reported the application of single-cell mRNA sequencing to identify a unique population of fibroblasts that exits in the fracture callus of bisphosphonate-treated rats. Such unique population of fibroblasts prevented fracture healing by secreting ECM. Further, it was found that these ECM-secreting fibroblasts were enriched for myeloid genes, suggesting a bone marrow orgin. After fractures were treated with local CGRP injection or Magnesium based biometal, such population of fibroblasts was cleared off, resulting in facilitated fracture healing of bisphosphonate-treated rats.
2022-02-03 | GSE185988 | GEO
Project description:The specific genes that distinguish normal fracture healing from abnormal healing or nonunion in humans are unknown. This study was an exploratory investigation of peripheral blood from acutely injured fracture patients collected over multiple days to compare normal healers, slow healers, and nonunion outcomes. We used microarrays to do a global comparison between acutely injured (AI) subjects (AIS) vs. age- and sex-matched healthy volunteers (HV) and chronic Nonunion (NU) patients.
2017-01-11 | GSE93378 | GEO