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Human oral metagenome

ABSTRACT: Influence of DNA extraction on oral microbial profiles obtained via 16S rRNA gene sequencing- Mock Communities

PROVIDER: PRJNA239464 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1177956.fastq.gz	Fastqsanger.gz
	SRR1177957.fastq.gz	Fastqsanger.gz
	SRR1177958.fastq.gz	Fastqsanger.gz
	SRR1177959.fastq.gz	Fastqsanger.gz

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human oral metagenome

Project description:Influence of DNA extraction on oral microbial profiles obtained via 16S rRNA gene sequencing- Supragingival plaque

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The mediating roles of the oral microbiome in saliva and subgingival sites between e-cigarette smoking and gingival inflammation

Project description:In this paper, we first report that EC smoking significantly increases the odds of gingival inflammation. Then, we seek to identify and explain the mechanism that underlies the relationship between EC smoking and gingival inflammation via the oral microbiome. We performed mediation analyses to assess if EC smoking affects the oral microbiome, which in turn affects gingival inflammation. For this, we collected saliva and subgingival samples from EC users and non-users and profiled their microbial compositions via 16S rRNA amplicon sequencing. We then performed α-diversity, β-diversity, and taxonomic differential analyses to survey the disparity in microbial composition between EC users and non-users. We found significant increases in α-diversity in EC users and disparities in β-diversity between EC users and non-users.

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Project description:Human Oral Cavity 16S rRNA metagenomic sequencing

| PRJNA198778 | ENA