Project description:The present study aims to assess the potential changes in microRNAs of proximal renal tubular cells in response to the adhesion of calcium oxalate monohydrate (COM) crystals. microRNA microarray was applied to evaluate the expression of HK-2 cells exposed to COM crystals for 0 and 24 hours.
Project description:The present study aims to assess the potential changes in LncRNAs of proximal renal cells in response to the adhesion of calcium oxalate monohydrate (COM) crystals. lncRNA microarray were applied to evaluate the expression of HK-2 cells exposed to COM crystal for 0 and 24 hours.
Project description:The present study aims to assess the potential changes in microRNAs of proximal renal tubular cells in response to the adhesion of calcium oxalate monohydrate (COM) crystals.
Project description:The present study aims to assess the potential changes in LncRNAs of proximal renal cells in response to the adhesion of calcium oxalate monohydrate (COM) crystals.
Project description:Analysis of altered LncRNA expression profiles in proximal renal tubular cells in response to calcium oxalate monohydrate crystal adhesion
| PRJNA245447 | ENA
Project description:Inhibition of NLRP3 alleviates calcium oxalate crystal-induced renal fibrosis and crystal adhesion
Project description:Various types of crystal can form in our bodies under normal and pathological conditions, with the more harmful of these crystals being studied intensively. However, much less is known about calcium oxalate dihydrate (COD) crystals, which are commonly associated with benign conditions. In general, calcium oxalates are pathological biominerals found in kidney stones and as microcalcifications (MCs) in breast and thyroid cancers. In breast cancer MCs, COD crystals are exclusively associated with benign lesions, and we have recently shown that they suppress breast precancer progression in vitro. Here, we studied the influence of synthetic calcium oxalate crystals – both COD and calcium oxalate monohydrate (COM) – with different morphologies and properties on crystal-cell interactions. We found that the less negatively charged crystals exhibited a greater tendency to aggregate and, as aggregates, attached better to cells, particularly to the cells of an invasive breast cancer cell line. We did not find any association between crystallinity, surface area, and solubilities of the crystals and attachment to cells. The crystal types that did attach efficiently to the cells, namely, COD with a thin or thick bipyramidal morphology, had the greatest impact on cell phenotype and functionally delayed cell growth, most probably through contact inhibition. Notably, in terms of morphology and size, these crystals resembled COD MCs found in benign breast tissues, suggesting that they are involved in a mechanism of tumor suppression.
Project description:parental HEK293T were challenged with 1064 µg/cm2 calcium oxalate monohydrate (COM) or sodium oxalate (NaOx) 4mM or vehicle for 24 hours and DNA microarray was performed. We observed and selected two-fold upregulation of laminin, beta 3 (LAMB3), early growth response 1 (EGR1), gremlin 1, DAN family BMP antagonist, Ca++-dependent secretion activator 2, Ras association domain family member 3, interleukin 33 and bone morphogenetic protein 8a , and two-fold down-regulation of interleukin 37 and intercellular adhesion molecule 1 in COM lord compared to vhicle.
Project description:Trigonelline is a phytoalkaloid commonly found in green and roasted coffee beans. It is also found in decaffeinated coffee. Previous report has shown that trigonelline-rich plant extract exhibits anti-lithiatic effects in a nephrolithiatic rat model. Nevertheless, cellular mechanisms underlying the anti-lithiatic properties of trigonelline remain hazy. Herein, we used nanoLC-ESI-Qq-TOF MS/MS and MaxQuant algorithm to perform label-free quantitative proteomics to identify trigonelline-induced changes in protein expression in MDCK renal cells. From a total of 1,006 and 1,011 proteins identified from control and trigonelline-treated cells, respectively, levels of 62 (23 up-regulated and 39 down-regulated) proteins were significantly changed by trigonelline. Functional enrichment and reactome pathway analyses showed that these 62 altered proteins were related to stress response, cell cycle and cell polarity. Functional validation by corresponding experimental assays revealed that trigonelline prevented calcium oxalate monohydrate crystal-induced renal cell deteriorations by inhibiting crystal-induced overproduction of intracellular reactive oxygen species, cell cycle shift from G0/G1 to G2/M phase, tight junction disruption, and epithelial-mesenchymal transition in renal epithelial cells. These findings provide cellular mechanisms and convincing evidence for the renoprotective effects of trigonelline, particularly in kidney stone prevention.
