Project description:FLP and MYB88 are two paralogous MYB proteins, regulating the symmetric division of guard mother cell during Arabidopsis stomatal development. To understand their molecular functions, we performed genome-wide identification of FLP/MYB88 binding targets using ChIP-chip with FLP/MYB88 antibody. By comparing ChIP-chip between wild-type and flp-1 myb88 lines, a total genes were identified as putative direct targets for FLP/MYB88.
Project description:FLP and MYB88 are two paralogous MYB proteins, regulating the symmetric division of guard mother cell during Arabidopsis stomatal development. To understand their molecular functions, we performed genome-wide identification of FLP/MYB88 binding targets using ChIP-chip with FLP/MYB88 antibody. By comparing ChIP-chip between wild-type and flp-1 myb88 lines, a total genes were identified as putative direct targets for FLP/MYB88. Ten-day old wild-type and flp-1 myb88 Arabidopsis green tissues were used for ChIP. Rabbit polyclonal antibody recognizing FLP and MYB88 proteins was used for chromatin immunoprecipitation
Project description:In land plants, guard cells sense pathogens and close stomata to resist their entry. Pathogen-infected local leaves transmit the danger status to uninfected distal systemic leaves and trigger their stomatal closure as a global defense termed systemic stomatal immunity. However, the corresponding mobile signals remain unknown. Here, we report that an upstream open reading frame (uORF) encodes a long-distance mobile peptide inducing systemic stomatal closure and named uORF-encoded systemic stomatal immune conductor (USIC). In local leaves, USIC expression is upregulated upon pathogen/pattern signals. In systemic leaves, the SIRK1 receptor and KIN7 coreceptor perceives USIC, and induces KIN7 cleavage by MC4. KIN7 translocates to tonoplasts and mediates stomatal closure by associating with H+-ATPases and aquaporins. This study reveals a systemic signaling mechanism whereby an uORF-encoded mobile peptide and its receptor pathway activating systemic stomatal immunity.