Project description:Peanut (Arachis hypogaea) has a large (~2.7 Gbp) allotetraploid genome with closely related component genomes making its genome very challenging to assemble. Here we report genome sequences of its diploid ancestors (A. duranensis and A. ipaënsis). We show they are similar to the peanutâs A- and B-genomes and use them use them to identify candidate disease resistance genes, create improved tetraploid transcript assemblies, and show genetic exchange between peanutâs component genomes. Based on remarkably high DNA identity and biogeography, we conclude that A. ipaënsis may be a descendant of the very same population that contributed the B-genome to cultivated peanut. Whole Genome Bisulphite Sequencing of the peanut species Arachis duranensis and Arachis ipaensis.
Project description:Peanut is one of the most important cash crops with high quality oil, high protein content, and many other nutritional elements, and grown globally. Cultivated peanut (Arachis hypogaea L.) is allotetraploid with a narrow genetic base, and its genetics and molecular mechanisms controlling the agronomic traits are poorly understood. The array SNP data was used for revaling of key candidate loci and genes associated with important agronomic traits in peanut
Project description:Peanut (Arachis hypogaea L.) is a prominent legume and oilseed crop cultivated globally for its economic significance and nutritional value, as it is rich in fat, protein, carbohydrates, and micronutrients. To meet the growing demand for edible oil and protein, it is necessary to further expand the cultivation area and improve the seed yield of peanut. Salinized land represents a potential resource for expanding peanut cultivation. However, most peanut genotypes are sensitive to salt stress, and soil salinity severely limits peanut productivity. Therefore, enhancing salt tolerance in peanut is essential. This study aimed to identify genes associated with salt stress through transcriptome analysis, thereby providing a genetic basis for improving salt tolerance in peanut. To identify salt stress-related genes, two cultivars—Huayu 33 (HY33, salt-tolerant) and Huayu 9115 (HY9115, salt-sensitive)—were subjected to salt stress treatment. Plants of HY33 and HY9115 were collected at 0 h, 3 h, 12 h, 24 h, and 48 h after salt treatment for transcriptome sequencing. Using the RNA sequencing data, numerous differentially expressed genes were identified as candidates for further functional study. This study provides extensive gene expression data for identifying key genes related to salt stress and establishes a basis for elucidating the underlying regulatory mechanisms. These findings can contribute to enhancing the salt tolerance of peanut through molecular breeding.
