Project description:Dendrobium officinale is a common and expensive traditional Chinese medicine used as a medicinal agent and food that has immunoregulatory and anti-inflammatory effects. Methods to authenticate D. officinale are lacking. Although chemical profiles of D. officinale were published, transcriptomic profiling is lacking. Here, we collected D. officinale from two regions (Yunnan and Miandian) and the analogue D. compactum. The Yunnan samples had three different quality levels. All samples were subjected to genome-wide biological response fingerprinting (BioReF) in RAW264.7 cells using RNA sequencing.
Project description:Dendrobium officinale is a traditional medicinal herb with a mount of bioactive components. Alkaloid is one of the major active ingredients of Dendrobium plants, and its immune regulatory effects have been well-studied. A total of 4857 DEGs, including 2943 up- and 1932 down-regulated genes, were identified between the control and MeJA-treated groups. Several shikimate and methylerythritol 4-phosphate pathway genes and a number of MeJA-induced P450 family genes, aminotransferase genes and methyltransferase genes were identified, providing several important candidates to further elucidate the alkaloid biosynthetic pathway of D. officinale. Furthermore, a large number of MeJA-induced transcript factor encoding genes were identified, suggesting a complex genetic network affecting the alkaloid metabolism in D. officinale.
