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Enterococcus faecalis

ABSTRACT: Genome-wide Analysis of Enterococcus faecalis OG1RF(pCF10) Gene Expression During Infection

PROVIDER: PRJNA252696 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1391146_1.fastq.gz	Fastqsanger.gz
	SRR1391146_2.fastq.gz	Fastqsanger.gz
	SRR1391147_1.fastq.gz	Fastqsanger.gz
	SRR1391147_2.fastq.gz	Fastqsanger.gz
	SRR1391148_1.fastq.gz	Fastqsanger.gz

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Genome-wide Analysis of Enterococcus faecalis OG1RF Gene Expression During Infection

Project description:Changes in Enterococcus faecalis OG1RF gene expression during infection in a rabbit model of subdermal abscess formation were studied using microarray analysis.

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Genome-wide Analysis of Enterococcus faecalis OG1RF(pCF10) Gene Expression During Infection

Project description:Changes in Enterococcus faecalis OG1RF(pCF10) gene expression at 4 hours post-infection in a rabbit model of subdermal abscess formation were studied using RNA-seq analysis.

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Project description:Quantitative analysis of mRNA levels in Enterococcus faecalis OG1RF across a growth time course in BHI media

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RNA-seq of phage VPE25 treated Enterococcus faecalis OG1RF relative to untreated controls

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Transcriptome analysis of Enterococcus faecalis OG1 delta-EF2638 mutant

Project description:Analysis of changes in gene expression in Enterococcus faecalis OG1 delta-EF2638 mutant compared to wild-type OG1 strain. The deletion mutant has a growth defect when grown with aeration The mutant presented in this study is described and characterized in Vesic, D. and Kristich, C.J. 2012. A Rex-family transcriptional repressor influnces H2O2 accumulation by Enterococcus faecalis. (submitted for publication) Microarray analysis was done using RNA isolated from two independent cultures of wild-type Enterococcus faecalis OG1 and two independent cultres of Enterococcus faecalis OG1 delta-EF2638 mutant; each RNA sample was subjected to triplicate hybridization (technical replicates) . Microarrays were custom designed to investigate expression of ORFs in Enterococcus faecalis OG1RF genome. The arrays were designed based on the OG1RF annotation generated with the Rapid Annotation Using Subsystem Technology (RAST) server (Aziz et. al. 2008. BMC Genomics 9:75), as described in Frank et al (2012) Infect. Immun. 80:539. The aim was eighteen probe pairs per ORF, each of which is present in triplicate.

2013-04-15 | E-GEOD-43228 | biostudies-arrayexpress

Genome-wide Analysis of Enterococcus faecalis OG1RF Gene Expression During Infection

Project description:Changes in Enterococcus faecalis OG1RF gene expression during infection in a rabbit model of subdermal abscess formation were studied using microarray analysis. Samples assayed in arrays consisted of the input inoculum and total RNA collected from the subdermal chambers at 2 and 8 hours post-inoculation. Alexa647-labeled samples were co-hybridized with 0.5 micrograms of sheared OG1RF genomic DNA labeled with Cy3 as a standard reference between chips. Four biological replicates were performed for each of the 3 time points.

2013-12-15 | E-GEOD-22391 | biostudies-arrayexpress

Enterococcus faecalis OG1RF

Project description:Genome-wide Analysis of Enterococcus faecalis OG1RF Gene Expression During Infection

| PRJNA127627 | ENA

Transcriptomes of Enterococcus faecalis OG1RF and OG1RF Δbph in planktonic culture

Project description:Liquid cultures of Enterococcus faecalis OG1RF and OG1RF Δbph were grown in tryptic soy broth without added dextrose (TSB-D) for 2 and 4 hr. At each time point, the transcriptomes were compared to identify differentially expressed genes in the Δbph mutant.

2022-03-12 | GSE198051 | GEO

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