Project description:To identify a novel miRNA that is aberrantly expressed in GICs, we analyzed differences in miRNA expression between the human GICs and glioma cell lines and neural stem cells by miRNA microarrays. We examined the miRNA expression profiles of five human GICs that were obtained from human glioma samples and two human glioma cell lines, U87 and U251, and NSC (neural stem cells) as a control.
Project description:Using Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array, we compared the gene expression profiles between the glioma cell lines U87 and Glio6, cultured at 3% of oxygene.
Project description:Comparison of temporal gene expression profiles to identify genes/pathways changing during ageing. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)
Project description:U87-MG human glioma cells were subjected to CRISPR/Cas9 genetic engineering to knock-out CHI3L1 gene. We then compared gene expression profiles between maternal cells (wild-type, WT) and two clones obtained throught CRISPR/Cas9 pipeline: clone 1 (KO1) and clone 2 (KO2)
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
Project description:The FAT1 gene was knocked down using 2 independent siRNAs, in immortalized human astrocytes and U87 and U251 glioma cell lines. A non-targeted scramble siRNA was used as a control.
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression.
