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Beauveria bassiana

ABSTRACT: Transcriptomic analysis of two 14-3-3 proteins Bmh1 and Bmh2 under osmotic and oxidative stresses in the entomopathogen fungus Beauveria bassiana by using RNA sequencing

PROVIDER: PRJNA253184 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1427291_1.fastq.gz	Fastqsanger.gz
	SRR1427291_2.fastq.gz	Fastqsanger.gz
	SRR1427292_1.fastq.gz	Fastqsanger.gz
	SRR1427292_2.fastq.gz	Fastqsanger.gz
	SRR1427293_1.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of LaeA and LaeB deletion strains in fungus Beauveria bassiana

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Transcriptomic analysis of LaeA and LaeB deletion strains in fungus Beauveria bassiana

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Grapevine response to Beauveria bassiana treatment

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Project description:Culturing the entomopathogenic fungus Beauveria bassiana under high glucose concentrations coupled with high aeration results in a fungal developmental shift from hyphae growth to mostly blastospores (yeast-like cells). The underlying molecular mechanisms involved in this shift remains elusive. Therefore, this has motivated us to realize a systematic analysis of the differential gene expression to uncover the fungal transcriptomic response to osmotic and oxidative stresses associated with the resulting high blastospores yield. Differential gene expression was compared under moderate glucose concentration (10% w/v) and high glucose concentration (20% w/v) daily for the three-day culturing. The RNAseq-based transcriptomic results underpinned a higher proportion of down- than up-regulated genes when the fungus was grown under 20% glucose than at 10%. A follow-up study explored a broader glucose range (4, 8, 12, 16, 20% w/v) with phenotype assessment and qRT-PCR of selected genes. Antioxidant, calcium transport, conidiation, and osmosensor related genes were highly up-regulated in higher glucose titers (18-20%) than at lower titers. These findings improved the knowledge of the molecular mechanisms during blastospore development and may help the large scale blastospores industrial production.

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Project description:Trancriptome of entomopathogen fungi Beauveria bassiana growing in host hemocoel

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Revealing the Immune Response of Sitona callosus to Beauveria bassiana Infection through Integrative Analyses of Transcriptomics and Metabolomics

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