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Mus musculus

ABSTRACT: Transcriptional response to stress in serum deprived mouse fibroblasts in the presence of MSK1/2 inhibitor.

PROVIDER: PRJNA253332 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1448830.fastq.gz	Fastqsanger.gz
	SRR1448831.fastq.gz	Fastqsanger.gz
	SRR1448832.fastq.gz	Fastqsanger.gz
	SRR1448833.fastq.gz	Fastqsanger.gz
	SRR1448834.fastq.gz	Fastqsanger.gz

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Mus musculus

Project description:Transcriptional response to stress in serum deprived mouse fibroblasts

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Transcriptional response to stress in serum deprived mouse fibroblasts in the presence of MSK1/2 inhibitor.

Project description:We have employed gene expression profiling in order to identify targets of transcriptional response to stress in resting mouse Swiss 3T3 fibroblasts, either untreated (control) or treated with anisomycin for 3 or 6 hours to induce the p38/MAP kinase pathway.Â In order determine transcriptional effects dependent on MSK1/2 kinase activity, H89 inhibitor was used in the study. Serum starved (72 h 0.2% FCS) mouse 3T3 cells were treated with anisomycin (188.5 nM) for 3 h or 6h (in duplicates) either with or without 15-min pre-treatment with MSK1/2 inhibitor H89 (10 uM). Untreated, serum-starved cells were used as a control. RNA was collected and gene expression profiling using strand-specific RNA-seq was performed.

2014-08-12 | E-GEOD-58746 | biostudies-arrayexpress

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Project description:Transcriptional response to stress in serum deprived mouse fibroblasts [RNA-Seq]

| PRJNA240991 | ENA

Transcriptional response to stress in serum deprived mouse fibroblasts in the presence of MSK1/2 inhibitor.

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Mus musculus

Project description:Promoter proximal pausing in response to stress in serum deprived mouse fibroblasts.

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Project description:Stress-induced signaling to the nucleosome in serum deprived mouse fibroblasts.

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Promoter proximal pausing in response to stress in serum deprived mouse fibroblasts.

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Immediate-Early and Delayed Primary Response Genes are Distinct in Function and Genomic Architecture

Project description:Transcriptional profiling time course of serum deprived quiescent human T98G cells follwing platelet derived growth factor (PDGF) stimulation in the absence and presence of the translation inhibitor, cycloheximide. RNA was harvested at 0.5, 2, and 4 hr following PDGF treatment. Keywords: time course

2007-07-03 | GSE8315 | GEO

Promoter proximal pausing in response to stress in serum deprived mouse fibroblasts.

Project description:We have employed short-capped RNA sequencing (sc-RNA-seq) in order to identify genes whose expression is regulated by promoter proximal pausing of RNA Polymerase II (RNAPI) in response to stress stimulation. We used serum-deprived mouse Swiss 3T3 fibroblasts, either untreated (control) or treated with anisomycin to induce the p38/MAP kinase pathway. Serum starved (72 h 0.2% FCS) mouse 3T3 cells were treated with anisomycin (188.5 nM) for 1 h (in duplicates). Untreated, serum-starved cells were used as a control. We isolated nuclear RNA, performed size fractionation followed by isolation of short-capped RNAs (scRNA). scRNAs were subsequently converted into DNA library and sequenced.

2014-08-12 | E-GEOD-55775 | biostudies-arrayexpress

Immediate-Early and Delayed Primary Response Genes are Distinct in Function and Genomic Architecture

2010-06-10 | E-GEOD-8315 | biostudies-arrayexpress