Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Arabidopsis thaliana

ABSTRACT: A transcriptional map following the developmental trajectory of the Arabidopsis stomatal lineage

OTHER RELATED OMICS DATASETS IN: GSE58857

PROVIDER: PRJNA253731 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR1463330.fastq.gz	Fastqsanger.gz
	SRR1463331.fastq.gz	Fastqsanger.gz
	SRR1463332.fastq.gz	Fastqsanger.gz
	SRR1463333.fastq.gz	Fastqsanger.gz
	SRR1463334.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 11

Similar Datasets

Arabidopsis thaliana

Project description:A transcriptional map following the developmental trajectory of the Arabidopsis stomatal lineage

| PRJNA253726 | ENA

Project description:A transcriptional map following the developmental trajectory of the Arabidopsis stomatal lineage

| PRJNA253729 | ENA

E-GEOD-58856

Project description:A transcriptional map following the developmental trajectory of the Arabidopsis stomatal lineage

2019-03-27 | E-GEOD-58856 | ExpressionAtlas

Warm temperature modifies lineage cell fates to reduce stomata production in Arabidopsis

Project description:Stomatal abundance decrease in Arabidopsis triggered by warm-temperature is attributed to PIF4-mediated repression of SPEECHLESS (SPCH) expression. We identified the unknown developmental and transcriptional basis of this adaptive response. We analyzed stomatal lineage progression at warm- and control-T combining live-cell imaging and lineage-cell tracing with epidermal phenotyping, genetics and transcriptomics. Warm-T or PIF4 overexpression caused a fraction of stomatal precursors to lose their identity and become diverted, explaining stomatal index reduction, and hinting at developmental mechanisms underlying lineage progression. Triggering diverted precursor fate required extended exposure to warm-T, below which the process is reversible and stomatal index remains unchanged. Despite heat-induced gene reprogramming silenced key positive drivers of stomatal development as SPCH and MUTE, most lineages progressed and formed stomata. Transcriptomics revealed that warm-T shifted lineages towards uncommitted cell stages, which regained committed fates during recovery at control temperature. This indicates that stomatal development under changing temperatures occurs through partly rewired gene circuits involving alternative pathways.

2025-06-24 | GSE278519 | GEO

Arabidopsis thaliana

Project description:Molecular profiling of stomatal lineage cell states

| PRJNA140931 | ENA

Single-Cell Resolution of Lineage Trajectories in the Arabidopsis Stomatal Lineage and Developing Leaf

Project description:Dynamic cell identities underlie flexible developmental programs. The stomatal lineages in the Arabidopsis leaf epidermis feature asynchronous and indeterminate divisions that can be modulated by environmental cues. The products of these lineages, stomatal guard cells and pavement cells, regulate plant-atmosphere exchanges, and the epidermis as a whole influences overall leaf growth. How flexibility is encoded in development of the stomatal lineage, and how cell fates are coordinated in the leaf are open questions. Here, we offer single-cell transcriptomes to uncover models of cell differentiation within Arabidopsis leaf tissue.

2021-04-05 | GSE167135 | GEO

A transcriptional map following the developmental trajectory of the Arabidopsis stomatal lineage

Project description:Developmental transitions can be described in terms of morphology and individual genes expression patterns, but also in terms of global transcriptional and epigenetic changes. Most of the large-scale studies of such transitions, however, have only been possible in synchronized cell culture systems. Here we generate a cell type specific transcriptome of an adult stem-cell lineage in the Arabidopsis leaf using RNA sequencing and microarrays. RNA profiles of stomatal entry, commitment, and differentiating cells, as well as of mature stomata and the entire aerial epidermis give a comprehensive view of the developmental progression.

2015-04-06 | GSE58855 | GEO

A transcriptional map following the developmental trajectory of the Arabidopsis stomatal lineage

2015-04-06 | GSE58857 | GEO

A transcriptional map following the developmental trajectory of the Arabidopsis stomatal lineage

2015-04-06 | GSE58856 | GEO

Co-immunoprecipitation of FAMA-interacting proteins in Arabidopsis thaliana seedlings

Project description:Identifying interaction partners and protein complex compositions for transcription factors (TFs) can produce valuable information on the mechanisms by which they regulate gene expression or are themselves regulated by signaling pathways in the cell. FAMA is a TF that is specifically expressed in the last stages of stomatal guard cell development in the epidermis of young leaves and other aerial tissues of higher plants. It is a master regulator of guard cell development and promotes terminal differentiation of the guard cell precursor by both activating and repressing hundreds of genes. How this is achieved mechanistically is still unclear. We therefore isolated putative FAMA interaction partners from young Arabidopsis seedlings expressing FAMA-CFP under the FAMA promoter using proteasome inhibitor treatment and formaldehyde crosslinking, followed by a classical co-immunoprecipitation mass spectrometry approach. Plants expressing nuclear GFP under a stomatal lineage specific promoter were used as controls. In two independent experiments with different crosslinking and immunoprecipitation conditions, we found ICE1, a known heterodimerization partner of FAMA, to be enriched in FAMA-CFP versus control samples. Other proteins with known transcriptional regulator or co-regulator function were not identified, presumably due to the low abundance of the bait protein. The interaction of FAMA with BRXL2, another regulator of guard cell development, which was detected in one experiment, is likely an artifact since BRXL2 does not localize to the nucleus under normal conditions.

2019-09-26 | PXD015212 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data