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Ovis aries

ABSTRACT: RNA seq analysis of day of pregnancy 14 ovine conceptuses

PROVIDER: PRJNA254105 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1503880.fastq.gz	Fastqsanger.gz
	SRR1503877.fastq.gz	Fastqsanger.gz
	SRR1503878.fastq.gz	Fastqsanger.gz
	SRR1503879.fastq.gz	Fastqsanger.gz

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RNA seq analysis of day of pregnancy 14 ovine conceptuses

Project description:RNA seq analysis was conducted to determine gene expression in the day 14 ovine conceptus. This was used in conjunction with the day 14 PPARG ChIP-seq analysis to identify genes bound by PPARG which were also expressed or not expressed in the day 14 conceptus. Understanding changes in gene expression during early pregnancy is critical to improving fertility and reproductive efficiency in ruminants. RNA seq analysis of 4 conceptuses from 4 individual Day 14 pregnant columbia/rambouillet crossbred ewes

2014-07-03 | E-GEOD-58967 | biostudies-arrayexpress

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Project description:PPARG regulated genes in the day 14 ovine conceptus

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PPARG regulated genes in the day 14 ovine conceptus

Project description:PPARG ChIP seq analysis was conducted to determine genes bound by and potentially regulated by PPARG in the developing ovine conceptus. Determination of gene regulation by prostaglandins through PPARG helps to improve our understanding of early pregnancy events and provides a basis for strategies to improve fertility and reproductive efficiency in ruminants. PPARG ChIP seq analysis of 4 conceptuses from 4 individual Day 14 pregnant columbia/ramboulette crossbred ewes

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Project description:Roadmap to implantation- RNA seq of ovine LE, GE and conceptus during early pregnancy

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Roadmap to implantation- RNA seq of ovine LE, GE and conceptus during early pregnancy

Project description:RNA seq analysis of laser capture microdissected luminal and glandular epithelium from ewes on day of pregnancy 10, 12, 14, 16 and 20. As well as RNA seq of whole conceptuses, and trophectoderm tissue from day 12, 14, 16 and 20 of pregnancy. Determination of gene expression changes in the uterine epithelium and conceptus during early pregnancy helps to improve our understanding of early pregnancy events and provides a basis of new strategies to improve fertility and reproductive efficiency in ruminants.

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Analysis of conceptuses from day 14 pregnancy rambouillet cross ewes

Project description:Analysis of conceptuses from day 14 pregnancy rambouillet cross ewes

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RNA seq analysis of day of pregnancy 14 ovine conceptuses

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Extracellular Vesicles Originate from the Conceptus and Uterus During Early Pregnancy in Sheep

Project description:The present studies tested the hypothesis that the elongating ovine conceptus and uterus produces EVs with the potential to mediate conceptus-maternal communication during early pregnancy. In Study One, EVs were purified from uterine luminal fluid (ULF) of day 14 cyclic sheep. The EVs were fluorescently labeled with PKH67 dye and infused into the uterine lumen of pregnant sheep for 6 days using an osmotic pump. On day 14, labeled EVs were observed in the conceptus trophectoderm and uterine epithelia, but not in the uterine stroma or myometrium. In Study Two, day 14 conceptuses were cultured ex vivo for 24 hours and found to release EVs into the culture medium. Isolated EVs from conceptuses were fluorescently labeled with PKH67 and infused into the uterine lumen of cyclic sheep for 6 days using an osmotic pump. On day 14, labeled EVs were observed in the uterine epithelia, but not in the uterine stroma or myometrium. No evidence of EV escape from the uterine lumen was observed by analysis of the ovary and other maternal tissues. Proteomics analysis of the day 14 conceptus-derived EVs identified 231 proteins that were enriched for extracellular space and several protein classes including proteases, protease inhibitors, chaperones and chaperonins. RNA-sequencing of day 14 conceptus-derived EVs detected expression of 512 mRNAs. The top expressed genes were overrepresented in ribosomal functions and components. These studies support the ideas that EVs emanate from both the conceptus trophectoderm and uterine epithelia and are involved in intercellular communication during the establishment of pregnancy. Transcriptional profiles from day 14 conceptus extracellular vesicles isolated from 24 hour conceptus-conditioned culture media (n=3) were generated by sequencing on the Illumina HiSeq 2500 platform.

2015-12-16 | E-GEOD-76035 | biostudies-arrayexpress

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Project description:Ovine footrot Metagenomics

| PRJNA933156 | ENA