Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Spiroplasma poulsonii

ABSTRACT: Spiroplasma poulsonii Genome sequencing and assembly

PROVIDER: PRJNA256019 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR4935236_subreads.fastq.gz	Fastqsanger.gz
	SRR5172866_subreads.fastq.gz	Fastqsanger.gz
	SRR6350066_subreads.fastq.gz	Fastqsanger.gz
	SRR8961770_subreads.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 5

Similar Datasets

Spiroplasma poulsonii

Project description:Spiroplasma poulsonii Genome sequencing and assembly

| PRJNA416288 | ENA

In Vitro Culture of the Insect Endosymbiont Spiroplasma poulsonii Highlights Bacterial Genes Involved in Host-Symbiont Interaction

Project description:Endosymbiotic bacteria associated with eukaryotic hosts are omnipresent in nature, particularly in insects. Studying the bacterial side of host-symbiont interactions is, however, often limited by the unculturability and genetic intractability of the symbionts. Spiroplasma poulsonii is a maternally transmitted bacterial endosymbiont that is naturally associated with several Drosophila species. S. poulsonii strongly affects its host’s physiology, for example by causing male killing or by protecting it against various parasites. Despite intense work on this model since the 1950s, attempts to cultivate endosymbiotic Spiroplasma in vitro have failed so far. Here, we developed a method to sustain the in vitro culture of S. poulsonii by optimizing a commercially accessible medium. We also provide a complete genome assembly, including the first sequence of a natural plasmid of an endosymbiotic Spiroplasma species. Last, by comparing the transcriptome of the in vitro culture to the transcriptome of bacteria extracted from the host, we identified genes putatively involved in host-symbiont interactions. This work provides new opportunities to study the physiology of endosymbiotic Spiroplasma and paves the way to dissect insect-endosymbiont interactions with two genetically tractable partners.

2018-03-24 | GSE112290 | GEO

Spiroplasma poulsonii

Project description:Spiroplasma poulsonii overview

| PRJNA427055 | ENA

"Blind-Killing" of male and female Drosophila embryos by a natural variant of the endosymbiotic bacterium Spiroplasma poulsonii

Project description:“Blind-Killing” of male and female Drosophila embryos by a natural variant of the endosymbiotic bacterium Spiroplasma poulsonii

2020-05-06 | GSE129674 | GEO

Spiroplasma poulsonii strain:sNeo

Project description:Spiroplasma poulsonii strain:sNeo Genome sequencing and assembly

| PRJNA492288 | ENA

Spiroplasma poulsonii isolate:neo

Project description:Spiroplasma poulsonii isolate:neo Genome sequencing and assembly

| PRJNA421356 | ENA

Drosophila hemolymph infected with Spiroplasma poulsonii

Project description:We investigated the effect of Spiroplasma infection on Drosophila hemolymph protein content using Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS). To this end, we extracted total hemolymph from uninfected and infected 10 days old females. At this age, Spiroplasma is already present at high titers in the hemolymph but does not cause major deleterious phenotypes to the fly. Extraction was achieved by puncturing the thorax and drawing out with a microinjector. Four replicates were made

2021-05-04 | PXD024340 | Pride

Spiroplasma phage MaM-2019a strain:Nebulosa Sex Ratio Organism

Project description:Spiroplasma poulsonii Bacteriophage-like Particle sequencing and assembly

| PRJNA545743 | ENA

Spiroplasma citri

Project description:Spiroplasma citri Genome sequencing and assembly

| PRJNA591027 | ENA

Spiroplasma melliferum IPMB4A

Project description:Spiroplasma melliferum IPMB4A Genome sequencing and assembly

| PRJNA80357 | ENA