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Osteoglossum bicirrhosum

ABSTRACT: Osteoglossum bicirrhosum Transcriptome

PROVIDER: PRJNA256921 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1532790_1.fastq.gz	Fastqsanger.gz
	SRR1532790_2.fastq.gz	Fastqsanger.gz
	SRR1532791_1.fastq.gz	Fastqsanger.gz
	SRR1532791_2.fastq.gz	Fastqsanger.gz
	SRR1532792_1.fastq.gz	Fastqsanger.gz

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Osteoglossum bicirrhosum

Project description:Osteoglossum bicirrhosum overview

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Osteoglossum bicirrhosum

Project description:Osteoglossum bicirrhosum Transcriptome or Gene expression

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Osteoglossum bicirrhosum

Project description:Osteoglossum bicirrhosum Genome sequencing and assembly

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Osteoglossum bicirrhosum strain:prfri_Ob_male_2019

Project description:Male Osteoglossum bicirrhosum Genome sequencing and assembly

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Osteoglossum bicirrhosum strain:prfri_Ob_female_2019 | breed:silver-arowana-female

Project description:Female Osteoglossum bicirrhosum Genome sequencing and assembly

| PRJNA881487 | ENA

Reference genome provide insights into sex determination of silver aworana (Osteoglossum bicirrhosum).

Project description:BackgroundSilver arowana (Osteoglossum bicirrhosum) is a basal fish species with sexual monomorphism, while its sex determination mechanism has been poorly understood, posing a significant challenge to its captive breeding efforts.ResultsWe constructed two high-quality chromosome-level genome assemblies for both female and male silver arowana, with scaffold N50 values over 10 Mb. Combining re-sequencing data of 109 individuals, we identified a female-specific region, which was localized in a non-coding region, i.e., around 26-kb upstream of foxl2 gene (encoding forkhead box L2). Its strong interaction with the neighboring foxl2 on the same chromosome suggests foxl2 as a candidate sex-related gene in silver arowana. We subsequently propose a complex gene network in the sex determination process of silver arowana, with foxl2 acting as the central contributor. Transcriptome sequencing of gonads support our hypothesis that the regulation of foxl2 can be influenced by the spatial proximity of the female-specific fragment, thereby promoting ovarian function or inhibiting testicular function to stimulate gonadal differentiation. Furthermore, we found the sex chromosomes to be homomorphic with a potentially recent origin, as a linkage disequilibrium analysis proved minor recombination suppression.ConclusionsThese results taken together serve as a crucial foundation for conducting extensive investigations on the evolution and differentiation of sex-determining mechanisms, as well as the emergence and development of sex chromosomes in various fishes.

| S-EPMC11776183 | biostudies-literature

Osteoglossum ferreirai isolate:CB-2025b

Project description:Osteoglossum ferreirai isolate:CB-2025b Genome sequencing

| PRJNA1328470 | ENA

Interspecific Genetic Differences and Historical Demography in South American Arowanas (Osteoglossiformes, Osteoglossidae, Osteoglossum).

Project description:The South American arowanas (Osteoglossiformes, Osteoglossidae, Osteoglossum) are emblematic species widely distributed in the Amazon and surrounding basins. Arowana species are under strong anthropogenic pressure as they are extensively exploited for ornamental and food purposes. Until now, limited genetic and cytogenetic information has been available, with only a few studies reporting to their genetic diversity and population structure. In the present study, cytogenetic and DArTseq-derived single nucleotide polymorphism (SNP) data were used to investigate the genetic diversity of the two Osteoglossum species, the silver arowana O. bicirrhosum, and the black arowana O. ferreirai. Both species differ in their 2n (with 2n = 54 and 56 for O. ferreirai and O. bicirrhosum, respectively) and in the composition and distribution of their repetitive DNA content, consistent with their taxonomic status as different species. Our genetic dataset was coupled with contemporary and paleogeographic niche modeling, to develop concurrent demographic models that were tested against each other with a deep learning approach in O. bicirrhosum. Our genetic results reveal that O. bicirrhosum colonized the Tocantins-Araguaia basin from the Amazon basin about one million years ago. In addition, we highlighted a higher genetic diversity of O. bicirrhosum in the Amazon populations in comparison to those from the Tocantins-Araguaia basin.

| S-EPMC6771150 | biostudies-literature

Intestinal Microbiome, Oral Microbiome, and Whole Blood Transcriptome Analyses in Gastrointestinal Malignancies

Project description:The primary objective of this prospective observational study is to characterize the gut and oral microbiome as well as the whole blood transcriptome in gastrointestinal cancer patients and correlate these findings with cancer type, treatment efficacy and toxicity. Participants will be recruited from existing clinical sites only, no additional clinical sites are needed.

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Surgical Treatment and Molecular Marker Exploration of Locally Recurrent Colorectal Cancer

Project description:This study intends to explore the clinicopathological characteristics and survival prognosis of locally recurrent colorectal cancer patients with different treatment modes by retrospectively analyzing the medical records of locally recurrent colorectal cancer patients who received hospitalization in our center. Transcriptome sequencing and public databases were used to screen for molecular markers related to locally recurrent colorectal cancer and to explore molecular markers’ regulatory role in the progression of locally recurrent colorectal cancer.

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