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Mus musculus

ABSTRACT: DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

PROVIDER: PRJNA257422 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1536140.fastq.gz	Fastqsanger.gz
	SRR1536135.fastq.gz	Fastqsanger.gz
	SRR1536136.fastq.gz	Fastqsanger.gz
	SRR1536137.fastq.gz	Fastqsanger.gz
	SRR1536138.fastq.gz	Fastqsanger.gz

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DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

Project description:We generated RRBS data to analyse the DNA methylation profiling among WT-AG-haESCs, DKO-AG-haESCs and round spermatids, we found deletion of H19 and Gtl2 DMRs do not change the methylation patterns in AG-haESCs base on all detected CpG sites. We used round spermatids as control and analysed the DNA methylation profiles of all the cell lines were by RRBS.

2015-06-29 | E-GEOD-60075 | biostudies-arrayexpress

DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

2015-06-29 | GSE60075 | GEO

Mus musculus

Project description:Gene expression analysis in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

| PRJNA257423 | ENA

Gene expression analysis in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

Project description:RNA seq result shows that WT-AG-haESCs and DKO-AG-haESCs samples are clustered together using hierarchical cluster both in the all expression genes and imprinting genes. This suggests that DKO of DMRs of H19 and Gtl2 do not change the overall gene expression patterns in AG-haESCs. We used round spermatids as control. Using RNA-seq, profile of all the expression genes and imprinting genes beteween different samples were analysed.

2015-06-29 | E-GEOD-60072 | biostudies-arrayexpress

Project description:Gene expression analysis and DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

| PRJNA257418 | ENA

Gene expression analysis in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

2015-06-29 | GSE60072 | GEO

Reduced diploidization and survival improvement of semi-cloned mice produced from androgenetic haploid embryonic stem cells

Project description:We found that the overall DNA methylation and hydroxymethylation in AG-haESCs are extremely low, and the downregulation of both de novo methyltransferase Dnmt3b and methylation maintenance enzyme Dnmt1was discovered responsible for this DNA hypomethylation. Further, our study discovered that the correction of DNA methylation can greatly reduce the incidence of diploidization and further improve the survival of semi-cloned mice produced from AG-haESCs.

2017-12-31 | GSE99588 | GEO

Mus musculus

Project description:Round Spermatids and Pachytene Spermatocytes

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Mus musculus

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and dKO round spermatids Transcriptomes

| PRJNA254123 | ENA

Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells

Project description:The use of two inhibitors of Mek1/2 and Gsk3β (2i) promotes the generation of mouse diploid and haploid embryonic stem cells (ESCs) from the inner cell mass of biparental and uniparental blastocysts, respectively. However, a system enabling long-term maintenance of imprints in ESCs has proven challenging. Here, we report that usage of a two-step a2i (alternative two inhibitors of Src and Gsk3β, TSa2i) derivation/culture protocol results in the establishment of androgenetic haploid ESCs (AG-haESCs) with stable DNA methylation at paternal DMRs (differentially DNA methylated regions) up to passage 60 that can efficiently support generating mice upon oocyte injection. We also show coexistence of H3K9me3 marks and ZFP57 bindings with intact DMR methylations. Furthermore, we demonstrate that TSa2i-treated AG-haESCs are a heterogeneous cell population regarding paternal DMR methylation. Strikingly, AG-haESCs with late passages display increased paternal-DMR methylations and improved developmental potential compared to early-passage cells, in part through the enhanced proliferation of H19-DMR hypermethylated cells. Together, we establish AG-haESCs that can long-term maintain paternal imprints.

2022-01-26 | GSE168447 | GEO

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