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MRNA destabilization is the dominant effect of mammalian microRNAs by the time substantial repression ensues (sequencing)

ABSTRACT: mRNA destabilization is the dominant effect of mammalian microRNAs by the time substantial repression ensues (sequencing)

OTHER RELATED OMICS DATASETS IN: GSE60426

PROVIDER: PRJNA258219 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1551120.fastq.gz	Fastqsanger.gz
	SRR1551130.fastq.gz	Fastqsanger.gz
	SRR1551140.fastq.gz	Fastqsanger.gz
	SRR1551150.fastq.gz	Fastqsanger.gz
	SRR1551160.fastq.gz	Fastqsanger.gz

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Similar Datasets

mRNA destabilization is the dominant effect of mammalian microRNAs by the time substantial repression ensues (sequencing)

Project description:MicroRNAs (miRNAs) regulate target mRNAs through a combination of translational repression and mRNA destabilization, with mRNA destabilization dominating at steady state in the few contexts examined globally. Here, we extend the global steady-state measurements to additional mammalian contexts and find that regardless of the miRNA, cell type, growth condition, or translational state, mRNA destabilization explains most (66%–>90%) miRNA-mediated repression. We also determine the relative dynamics of translational repression and mRNA destabilization for endogenous mRNAs as a miRNA is induced. Although translational repression occurs rapidly, its effect is relatively weak, such that by the time consequential repression ensues, the effect of mRNA destabilization dominates. These results imply that consequential miRNA-mediated repression is largely irreversible and provide other insights into the nature of miRNA-mediated regulation. They also simplify future studies, dramatically extending the known contexts and time points for which monitoring mRNA changes captures most of the direct miRNA effects.

2014-09-25 | GSE60426 | GEO

mRNA destabilization is the dominant effect of mammalian microRNAs by the time substantial repression ensues (gene expression)

Project description:MicroRNAs (miRNAs) regulate target mRNAs through a combination of translational repression and mRNA destabilization, with mRNA destabilization dominating at steady state in the few contexts examined globally. Here, we extend the global steady-state measurements to many additional mammalian contexts and find that regardless of the miRNA, cell type, growth condition or translational state, mRNA destabilization explains most (70% to >90%) miRNA-mediated repression. We also determine the relative dynamics of translational repression and mRNA destabilization for endogenous mRNAs as a miRNA is induced. Although translational repression occurs rapidly, its effect on gene expression is relatively weak, such that by the time consequential repression ensues, the effect of mRNA destabilization dominates. These results add to the fundamental understanding of miRNAs, imply that consequential miRNA-mediated repression is largely irreversible and simplify future studies, dramatically extending the known contexts and time points for which monitoring mRNA changes captures most of the direct miRNA effects. 6 samples from a variety of primary cell types

2014-09-25 | E-GEOD-60976 | biostudies-arrayexpress

mRNA destabilization is the dominant effect of mammalian microRNAs by the time substantial repression ensues (gene expression)

Project description:MicroRNAs (miRNAs) regulate target mRNAs through a combination of translational repression and mRNA destabilization, with mRNA destabilization dominating at steady state in the few contexts examined globally. Here, we extend the global steady-state measurements to many additional mammalian contexts and find that regardless of the miRNA, cell type, growth condition or translational state, mRNA destabilization explains most (70% to >90%) miRNA-mediated repression. We also determine the relative dynamics of translational repression and mRNA destabilization for endogenous mRNAs as a miRNA is induced. Although translational repression occurs rapidly, its effect on gene expression is relatively weak, such that by the time consequential repression ensues, the effect of mRNA destabilization dominates. These results add to the fundamental understanding of miRNAs, imply that consequential miRNA-mediated repression is largely irreversible and simplify future studies, dramatically extending the known contexts and time points for which monitoring mRNA changes captures most of the direct miRNA effects.

2014-09-25 | GSE60976 | GEO

mRNA destabilization is the dominant effect of mammalian microRNAs by the time substantial repression ensues.

Project description:MicroRNAs (miRNAs) regulate target mRNAs through a combination of translational repression and mRNA destabilization, with mRNA destabilization dominating at steady state in the few contexts examined globally. Here, we extend the global steady-state measurements to additional mammalian contexts and find that regardless of the miRNA, cell type, growth condition, or translational state, mRNA destabilization explains most (66%->90%) miRNA-mediated repression. We also determine the relative dynamics of translational repression and mRNA destabilization for endogenous mRNAs as a miRNA is induced. Although translational repression occurs rapidly, its effect is relatively weak, such that by the time consequential repression ensues, the effect of mRNA destabilization dominates. These results imply that consequential miRNA-mediated repression is largely irreversible and provide other insights into the nature of miRNA-mediated regulation. They also simplify future studies, dramatically extending the known contexts and time points for which monitoring mRNA changes captures most of the direct miRNA effects.

| S-EPMC4292926 | biostudies-literature

mRNA destabilization is the dominant effect of mammalian microRNAs by the time substantial repression ensues

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-09-25 | GSE61073 | GEO

mRNA destabilization is the dominant effect of mammalian microRNAs by the time substantial repression ensues

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-09-25 | E-GEOD-61073 | biostudies-arrayexpress

Project description:mRNA destabilization is the dominant effect of mammalian microRNAs by the time substantial repression ensues

| PRJNA260185 | ENA

Destabilization of AETFC through C/EBPalpha-mediated repression of LYL1 contributes to t(8;21) leukemic cell differentiation

Project description:In t(8;21) leukemic cells, the leukemogenic fusion protein AML1-ETO is stabilized and functions through the AML1-ETO-containing transcription factor complex (AETFC). Destabilization of AETFC thus provides a strategy to target AML1-ETO. In this study, we found that AETFC can be destabilized by a specific mechanism involving a direct repression of the core component LYL1 by C/EBPalpha at transcriptional level. We performed a ChIP-seq analysis of the genome-wide occupancy of C/EBPalpha and identifeid a -1 kb C/EBPalpha-binding site in the LYL1 locus that mediate this repression. As LYL1 acts as a linker between the AML1-ETO-E and LMO2-LDB1 parts of AETFC, depletion of LYL1 causes a destabilization of AETFC, which increases susceptibility of the leukemic cells to differentiation. Our results have provided a novel mechanism by which C/EBPalpha can directly destabilize AETFC, and have identified LYL1 as a new therapeutic target for treatment of t(8;21) leukemia.

2019-02-19 | GSE114642 | GEO

Mus musculus

Project description:mRNA destabilization is the dominant effect of mammalian microRNAs by the time substantial repression ensues (gene expression)

| PRJNA260030 | ENA

Widespread destabilization of C. elegans microRNAs by the E3 ubiquitin ligase EBAX-1 [RNA-Seq]

Project description:MicroRNAs (miRNAs) associate with Argonaute (AGO) proteins to form a complex that directs mRNA repression. One way that miRNAs are regulated is at the level of their stability. Some animal miRNAs are destabilized through a pathway in which pairing to specialized RNA transcripts recruits the ZSWIM8 E3 ubiquitin ligase, which polyubiquitinates AGO, leading to its degradation and exposure of the miRNA to cellular nucleases. We found that 22 miRNAs in C. elegans are sensitive to loss of EBAX-1, the ZSWIM8 ortholog in nematodes, implying that these 22 miRNAs might be subject to this pathway of target-directed miRNA degradation (TDMD). These miRNAs included the embryonic miR-35–42 family, as well as other miRNAs among the least stable in the worm, suggesting that TDMD is a major pathway of miRNA destabilization in the worm. The excess miR-35–42 molecules that accumulated in ebax-1 mutants caused increased repression of their predicted target mRNAs and underwent 3′ trimming over time. In general, however, miRNAs sensitive to loss of EBAX-1 did not have a consistent pattern of either trimming or tailing. A previous study reports that in contrast to TDMD substrates in mammals and flies, the 3′ region of miR-35 is not required for its destabilization, raising the question of whether the 3′ regions of other miRNAs are dispensable for EBAX-1 sensitivity in nematodes. To address this question, we replaced the 3′ region of another EBAX-sensitive miRNA, miR-43, and found that this replacement greatly reduced EBAX-sensitivity in the worm. Together, these findings broaden the implied biological scope of TDMD-like regulation of miRNA stability in animals, and indicate that the requirement for miRNA 3′ sequences in this process is variable in the worm.

2024-11-04 | GSE267368 | GEO

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