Project description:We identified genes regulated by parasitization of the silkworm Bombyx mori by three tachinid parasitoid species, Exorista japonica, Drino inconspicuoides and Pales pavida, using oligonucleotide microarrays. The numbers of genes and their intensity of expression varied with the species of parasitoid, within silkworm hemocytes and fat body. Bombyx mori hemocyte, silkgland and fat body samples parasitizated by Exorista japonica, Drino inconspicuoides and Pales pavida were prepared. Gene expression was compared in these two groups: control and parasitized.
Project description:We identified genes regulated by parasitization of the silkworm Bombyx mori by three tachinid parasitoid species, Exorista japonica, Drino inconspicuoides and Pales pavida, using oligonucleotide microarrays. The numbers of genes and their intensity of expression varied with the species of parasitoid, within silkworm hemocytes and fat body.
Project description:Cell differentiation, tissue development and functional formation rely on the precise activation of specific gene expression which regulated by the interaction of epigenetic modifications and transcription factors. DNA G-quadruplex structures (G4s) have emerged as important regulators of gene transcription. Here, by nuclear G4-CUT&Tag, ATAC-seq, and histone modification data, we demonstrate that G4s in Bombyx mori are primarily located in promoter regions and are associated with active chromatin marks. Comparative analyses of G4 distribution in the brain and fat body revealed that identical promoter sequences can adopt distinct G4 folding states in different tissues. Notably, the presence of folded G4s strongly correlated with high transcriptional activity of genes essential for tissue-specific functions. Furthermore, treatment with G4-stabilizing ligands disrupted energy metabolism and significantly delayed neurodevelopment in B. mori. In summary, our findings emphasize G4s as critical epigenetic regulators in maintaining tissue development and functional formation in insect.
Project description:Cell differentiation, tissue development and functional formation rely on the precise activation of specific gene expression which regulated by the interaction of epigenetic modifications and transcription factors. DNA G-quadruplex structures (G4s) have emerged as important regulators of gene transcription. Here, by nuclear G4-CUT&Tag, ATAC-seq, and histone modification data, we demonstrate that G4s in Bombyx mori are primarily located in promoter regions and are associated with active chromatin marks. Comparative analyses of G4 distribution in the brain and fat body revealed that identical promoter sequences can adopt distinct G4 folding states in different tissues. Notably, the presence of folded G4s strongly correlated with high transcriptional activity of genes essential for tissue-specific functions. Furthermore, treatment with G4-stabilizing ligands disrupted energy metabolism and significantly delayed neurodevelopment in B. mori. In summary, our findings emphasize G4s as critical epigenetic regulators in maintaining tissue development and functional formation in insect.
Project description:In the silkworm, Bombyx mori, juvenile hormone (JH) and 20-hydroxyecdysone (20E) levels are high during the final larval molt (4M) but both absent during the feeding stage of 5th instar (5F), while JH level is low and 20E level is high during the prepupal stage (PP). Fat body is the important organs in insect, we want to find out differentially expressed genes which are respectively regulated by the two hormones. Total RNA from 4th molting,5th feeding and prepupa stages Bombyx fat body were used to generate target cDNA, and then hybridized to 48k Bombyx genome Array Genechips, representing about 23000 characterized genes
