Project description:Analysis of mechano-regulation of eIF2α phosphatases in multiple myeloma cells at gene expression level. The hypothesis tested in the present study was Guanbenz (GBZ) and Sal003, 2 different eIF2α phosphatases induce different responses in multiple myeloma cells . Results provide important information of the responses of multiple myeloma cells, such as specific mechano-responsive genes, up- or down-regulated specific cellular functions
Project description:Histone deacetylase (HDAC) inhibitors have been reported to exert synergistic antitumor effects with immunomodulatory drugs (IMiDs) in multiple myeloma, although the underlying molecular mechanisms are not fully elucidated. To delineate pathways potentially involved in this synergy, we profiled gene expression changes induced by HDAC inhibition in the OPM2 multiple myeloma cell line. Cells were treated with panobinostat (10 nM), romidepsin (10 nM), or ricolinostat (100 nM) for 2, 5, or 20 hours, followed by RNA-seq analysis. Two independent biological replicates were generated for each condition. These data provide a resource for identifying HDAC inhibitor–responsive genes and pathways that may contribute to the cooperative activity of HDAC inhibitors and IMiDs in multiple myeloma.
