Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA261631 | ENA |
REPOSITORIES: ENA
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Project description:Macromolecular trafficking and cell-to-cell communication in plants occurs via plasmodesmata (PD). Currently, little is known about the proteins defining these sophisticated cell-to-cell contacts. To uncover proteins required for PD structure and function we made use of the 17 kDa movement protein (MP17) of the Potato leafroll virus (PLRV). The protein is required for cell-to-cell movement of the virus and localizes specifically to branched PD in source tissues. By forward genetic screening for Arabidopsis mutants with altered PD binding of MP17, several mutants were found. Map-based cloning of one of these mutants revealed a mutation in the choline transporter-like 1 (CHER1) protein. The mutation abolished plasma membrane localization of the protein. As consequence phosphatidylcholine levels and PD binding of MP17 decreased. Transcriptome analysis revealed a down-regulation of defense genes and genes involved in the synthesis of very long chain fatty acids, indicating an impairment of lipid signaling. Furthermore, cher1 mutants showed a reduced assimilate export and stunted growth. These findings highlight the emerging role of phospholipids, especially in the context of PD structure and function as well as potential binding sites for plasma membrane- and PD-associated proteins, which has been neglected for a long time.
2016-06-01 | GSE61595 | GEO
Project description:Macromolecular trafficking and cell-to-cell communication in plants occurs via plasmodesmata (PD). Currently, little is known about the proteins defining these sophisticated cell-to-cell contacts. To uncover proteins required for PD structure and function we made use of the 17 kDa movement protein (MP17) of the Potato leafroll virus (PLRV). The protein is required for cell-to-cell movement of the virus and localizes specifically to branched PD in source tissues. By forward genetic screening for Arabidopsis mutants with altered PD binding of MP17, several mutants were found. Map-based cloning of one of these mutants revealed a mutation in the choline transporter-like 1 (CHER1) protein. The mutation abolished plasma membrane localization of the protein. As consequence phosphatidylcholine levels and PD binding of MP17 decreased. Transcriptome analysis revealed a down-regulation of defense genes and genes involved in the synthesis of very long chain fatty acids, indicating an impairment of lipid signaling. Furthermore, cher1 mutants showed a reduced assimilate export and stunted growth. These findings highlight the emerging role of phospholipids, especially in the context of PD structure and function as well as potential binding sites for plasma membrane- and PD-associated proteins, which has been neglected for a long time. light exposed source leaves from wild type Col-0, T-DNA insertion line cher1-4 and mutant cher1-5 (10 weeks old). Samples: per pool 2 source leaves from 4 different plants, taken in the afternoon; wildtype (WT): Col-0, cher1-4: T-DNA insertion line SALK-065853 (ecotype Col-0, kanamycin resistance), cher1-5: Col-0 with GGA -->GAA at position 740 of cds
2016-06-01 | E-GEOD-61595 | biostudies-arrayexpress
Project description:CHOLINE TRANSPORTER LIKE1 is required for sieve plate development to mediate long distance cell-to-cell communication
| PRJEB6417 | ENA
Project description:Phloem localization of plant viruses is advantageous for acquisition by sap-sucking vectors but hampers host-virus protein interaction studies. In this study, Potato leafroll virus (PLRV)-host protein complexes were isolated from systemically infected potato, a natural host of the virus. Comparing two different co-immunoprecipitation support matrices coupled to mass spectrometry, we identified 44 potato proteins and one viral protein (P1) specifically associated with virus isolated from infected phloem. An additional 142 proteins interact in complex with virus at varying degrees of confidence. Greater than 80% of these proteins were previously found to form high confidence interactions with PLRV isolated from the model host Nicotiana benthamiana. Bioinformatics revealed that these proteins are enriched for functions related to plasmodesmata, organelle membrane transport, translation and mRNA processing. Our results show that model system proteomics experiments are extremely valuable for understanding protein interactions regulating infection in recalcitrant pathogens such as phloem-limited viruses.
2016-12-14 | PXD004814 | Pride
Project description:<p><strong>INTRODUCTION:</strong> Grafting is a traditional research and plant production technique used to study the long-distance movement of molecules and reduce disease susceptibility, respectively. Tomato/potato grafts are rare examples of successful interspecies grafting, even resulting in commercially available products. Nevertheless, information on the effect of tomato on the quality parameters of potato tubers is scarce.</p><p><strong>MATERIALS AND METHODS:</strong> The tomato cultivar ‘Mobil’ was grafted with the potato cultivars ‘White Lady’, ‘Hópehely’ and ‘Désirée’, and the phenotype, metabolite composition, and starch and protein contents of tubers were analysed. Anthocyanins were isolated from the tuber skins, and the expression level of the transcription factor ANTHOCYANIN1 (StAN1) was evaluated.</p><p><strong>RESULTS:</strong> Out of the 112 identified metabolites, the concentrations of twelve compounds were altered in the same direction in all three cultivars. The starch content of tubers was increased, while the protein level remained unaltered in ‘White Lady’ and ‘Hópehely’. The oval tubers became roundish. The tomato scion increased the anthocyanin content of ‘Hópehely’ and ‘Désirée’ tuber skins, which was correlated with the upregulation of StAN1 expression.</p><p><strong>CONCLUSION:</strong> Tomato has a significant impact on the quality parameters of potato tubers and reduces the asparagine concentration and risk of acrylamide formation.</p>
2025-02-24 | MTBLS7601 | MetaboLights
Project description:Integrative genetic analysis identified FLVCR1 as a choline transporter in mammalian cells. We profilied the transcriptional response of HeLa cells with and without FLVCR1 to choline depeleted or replete conditions.
2023-08-08 | GSE228126 | GEO
Project description:Potato genotypes from a diploid potato population were divided in two groups based on their response to Potato virus A (PVA). Plants exhibiting hypersensitive response were compared to plants exhibiting non-necrotic response (i.e. blocking virus movement without cell death).<br>The comparisons were made before inoculation and 12 and 24 hours post-inoculation.<br>
2010-09-30 | E-MEXP-2622 | biostudies-arrayexpress
Project description:Genome-wide knock out screen identified members of the SLC25 family of mitochondrial carrier proteins as important regulators of the rate of de novo mitochondrial protein synthesis. To elucidate this relationship, we generated human cell knockouts for SLC25A25, SLC25A44, SLC25A45, and SLC25A48, which have been shown to exchange ATP and magnesium, branched-chain amino acids, methylated basic amino acids, and choline, respectively. Multiomic and functional analyses identified that these four carriers are crucial for mitochondrial translation, biogenesis and function of the oxidative phosphorylation system, as well as mitochondrial morphology. Thermostability screens showed that SLC25A48 is specifically stabilised by choline and changes in the mitochondrial metabolome and lipidome indicated defects in choline biosynthetic pathways and remodelling of mitochondrial membranes, both consistent with SLC25A48 being a choline transporter. These results highlight the essential roles of specific SLC25 transporters in maintaining mitochondrial structure and function, and show that of mitochondrial translation is sensitive to local concentrations of branched chain amino acids, methylated basic amino acids, ATP, magnesium and choline.
2025-12-19 | PXD066637 | Pride