Colletotrichum falcatum
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA262596 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR1765657_1.fastq.gz | Fastqsanger.gz | |||
| SRR1765657_2.fastq.gz | Fastqsanger.gz | |||
| SRR2557588_1.fastq.gz | Fastqsanger.gz | |||
| SRR2557588_2.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 21 |
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Project description:Colletotrichum falcatum strain:Cf08 Genome sequencing and assembly
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Project description:Colletotrichum falcatum strain:Cf671 Genome sequencing and assembly
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Project description:Colletotrichum falcatum MAFF306170
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Project description:Colletotrichum falcatum MAFF306170
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Project description:Colletotrichum falcatum Went causes red rot disease in sugarcane farming in the tropical and sub-tropical regions. This disease causes significant economic loss to the sugarcane production industry. Successful disease management strategies depend on understanding the evolutionary relationship between pathogens, genetic diversity, and population structure, particularly at the intra-specific level. Forty-one isolates of C. falcatum were collected from different sugarcane farms across Bangladesh for molecular identification, phylogeny and genetic diversity study. The four genes namely, ITS-rDNA, β-tubulin, Actin and GAPDH sequences were conducted. All the 41 C. falcatum isolates showed a 99-100% similarity index to the conserved gene sequences in the GenBank database. The phylogram of the four genes revealed that C. falcatum isolates of Bangladesh clustered in the same clade and no distinct geographical structuring were evident within the clade. The four gene sequences revealed that C. falcatum isolates from Bangladesh differed from other countries´ isolates because of nucleotides substitution at different loci. The genetic structure of C. falcatum isolates were determined using ISSR marker generated 404 polymorphic loci from 10 selected markers. The percentage of polymorphic loci was 99.01. The genetic variability at species level was slightly higher than at population level. Total mean gene diversity at the species level was 0.1732 whereas at population level it was 0.1521. The cluster analysis divided 41 isolates into four main genetic groups and the principal component analysis was consistent with cluster analysis. To the best of our knowledge, this is the first finding on characterizing C. falcatum isolates infesting sugarcane in Bangladesh. The results of this present study provide important baseline information vis a vis C. falcatum phylogeny analysis and genetic diversity study.
| S-EPMC8467384 | biostudies-literature
Project description:We evaluated transgenic lines of sugarcane modified with the barley chitinase class-II gene to create resistance against the red rot causative agent Colletotrichum falcatum Went. Local sugarcane cultivar SP93 was transformed with a 690-bp coding sequence of the chitinase-II gene under the influence of a polyubiquitin promoter. Transgenic sugarcane lines (T 0) overexpressing the chitinase gene were obtained through a particle bombardment method with 13.3% transformation efficiency. Four transgenic sugarcane lines, SCT-03, SCT-05, SCT-15, and SCT-20, were tested for resistance against red rot by in vitro antifungal assays. Crude protein extracts from transgenic sugarcane plants SCT-03, SCT-05, SCT-15, and SCT-20 inhibited the mycelial growth of C. falcatum by 49%, 40%, 56%, and 52%, respectively, in a quantitative in vitro assay. Our findings revealed that two transgenic lines, SCT-15 and SCT-20, exhibited the highest endochitinase activity of 0.72 and 0.58 U/mL, respectively. Furthermore, transgenic lines SCT-15 and SCT-20 exhibited strong resistance against inoculated C. falcatum in an in vitro bioassay, as they remained healthy and green in comparison with the control sugarcane plants, which turned yellow and eventually died 3 weeks after infection. The mRNA expression of the transgene in the C. falcatum-inoculated transgenic sugarcane lines increased gradually compared to the control plant. The mRNA expression was the highest at 72 h in both transgenic lines and remained almost stable in the subsequent hours.
| S-EPMC6353297 | biostudies-literature
Project description:The project is aimed at analysing the comparitive proteomics of red rot pathogen, C. falcatum, during red rot infection in sugarcane. The differentially abundant proteins shall be used to identify the corresponding genes.
2023-03-23 | PXD033368 | Pride
Project description:A total of 226 sugarcane rhizosphere-associated bacterial strains from the six different cultivars were screened against three pathogenic strains of C. falcatum (cfNAV, cfCHA, and cf8436) for the suppression of red rot disease. On the basis of mycelial growth inhibition in dual culture assay, 26 bacteria were selected for further characterization of morphology, biochemical activity, plant-growth-promoting (PGP) activity, antifungal potential and molecular identity by 16S rRNA gene sequence. On the basis of the 16S rRNA gene sequencing, it was found that the isolates belonged to proteobacteria (13), Firmicutes (10), and Bacteroides (3). The antagonistic bacteria tested for PGP traits revealed that 10 strains were able to solubilize tricalcium phosphate, 11 strains were able to produce siderophore, and 14 strains were able to grow in the N-free medium. The quantitative estimation of indole-3-acetic acid production was ranged from 21.58 to 66.31 μg/mL. On the basis of PGP and biocontrol traits, five strains Ochrobactrum intermedium (TRD14), Acinetobacter sp. (PK9), Bacillus sp. (RSC29 and KR91) and Escherichia sp. (VRE34) were further chosen for pot trial under greenhouse conditions on highly susceptible variety CoC671. The results showed that the pathogen-inoculated sugarcane plants were able to germinate but died within one month. However, the CoC671 inoculated with selected biocontrol strains found protected from disease and an increase in plant growth parameters on par with carbendazim fungicides. This study proves that the isolates identified in this study could be used as an alternative to chemical fungicides to control red rot pathogen of sugarcane plants.
| S-EPMC6389795 | biostudies-literature
Project description:Plants respond to pathogens and insect attacks by inducing and accumulating a large set of defense-related proteins. Two homologues of a barley wound-inducible protein (BARWIN) have been characterized in sugarcane, SUGARWIN1 and SUGARWIN2 (sugarcane wound-inducible proteins). Induction of SUGARWINs occurs in response to Diatraea saccharalis damage but not to pathogen infection. In addition, the protein itself does not show any effect on insect development; instead, it has antimicrobial activities toward Fusarium verticillioides, an opportunistic fungus that usually occurs after D. saccharalis borer attacks on sugarcane. In this study, we sought to evaluate the specificity of SUGARWIN2 to better understand its mechanism of action against phytopathogens and the associations between fungi and insects that affect plants. We used Colletotrichum falcatum, a fungus that causes red rot disease in sugarcane fields infested by D. saccharalis, and Ceratocystis paradoxa, which causes pineapple disease in sugarcane. We also tested whether SUGARWIN2 is able to cause cell death in Aspergillus nidulans, a fungus that does not infect sugarcane, and in the model yeast Saccharomyces cerevisiae, which is used for bioethanol production. Recombinant SUGARWIN2 altered C. falcatum morphology by increasing vacuolization, points of fractures and a leak of intracellular material, leading to germling apoptosis. In C. paradoxa, SUGARWIN2 showed increased vacuolization in hyphae but did not kill the fungi. Neither the non-pathogenic fungus A. nidulans nor the yeast S. cerevisiae was affected by recombinant SUGARWIN2, suggesting that the protein is specific to sugarcane opportunistic fungal pathogens.
| S-EPMC3946703 | biostudies-literature