Halobacterium salinarum NRC-1
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ABSTRACT:
PROVIDER: PRJNA263042 | ENA |
REPOSITORIES: ENA
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Project description:Evolution of Context Dependent Regulation by Expansion of Feast/Famine Regulatory Proteins [expression]
| PRJNA262850 | ENA
Project description:Antisense RNAs (asRNAs) are present in diverse organisms and play important roles in gene regulation. In this work, we mapped the primary antisense transcriptome in the halophilic archaeon Halobacterium salinarum NRC-1. By reanalyzing publicly available data, we mapped antisense transcription start sites (aTSSs) and inferred the probable 3' ends of these transcripts. We analyzed the resulting asRNAs according to the size, location, function of genes on the opposite strand, expression levels and conservation. We show that at least 21% of the genes contain asRNAs in H. salinarum. Most of these asRNAs are expressed at low levels. They are located antisense to genes related to distinctive characteristics of H. salinarum, such as bacteriorhodopsin, gas vesicles, transposases and other important biological processes such as translation. We provide evidence to support asRNAs in type II toxin⁻antitoxin systems in archaea. We also analyzed public Ribosome profiling (Ribo-seq) data and found that ~10% of the asRNAs are ribosome-associated non-coding RNAs (rancRNAs), with asRNAs from transposases overrepresented. Using a comparative transcriptomics approach, we found that ~19% of the asRNAs annotated in H. salinarum belong to genes with an ortholog in Haloferax volcanii, in which an aTSS could be identified with positional equivalence. This shows that most asRNAs are not conserved between these halophilic archaea.
| S-EPMC6523106 | biostudies-literature
Project description:ChIP-Chip of General Transcription factors in Halobacterium NRC-1
| PRJNA114681 | ENA
Project description:Halobacterium salinarum NRC-1 VNG1451C transcription factor ChIP-chip with and without carbon sources
| PRJNA110093 | ENA
Project description:Halobacterium salinarum NRC-1 conditional ChIP-chip for transcription initiation factor IIB 4 (TFBd)
| PRJNA114073 | ENA
Project description:Halobacterium sp. NRC-1 ChIP-chip for TFBa, TFBd and TFBf, high resolution array
| PRJNA116863 | ENA
Project description:Two transcription factors are necessary for iron homeostasis in a salt-dwelling archaeon [ChIP-chip data]
| PRJNA153855 | ENA
Project description:Iron and Acid Adapted Strains of Halobacterium sp. NRC-1 obtained by Experimental Evolution
| PRJNA541441 | ENA
Project description:Sustained bioenergy production from organisms that thrive in high salinity, low oxygen, and low nutrition levels is useful in monitoring hypersaline polluted environments. Microbial fuel cell (MFC) studies utilizing single species halophiles under salt concentrations higher than 1 M and as a closed microbial system are limited. The current study aimed to establish baseline voltage, current, and power density from a dual-chambered MFC utilizing the halophile Halobacterium salinarum NRC-1. MFC performance was determined with two different electrode sizes (5 cm2 and 10 cm2), under oscillating and nonoscillating conditions, as well as in a stacked series. A closed dual-chamber MFC system of 100 mL capacity was devised with Halobacterium media (4.3 M salt concentration) as both anolyte and catholyte, with H. salinarum NRC-1 being the anodic organism. The MFC measured electrical output over 7, 14, 28, and 42 days. MFC output increased with 5 cm2 sized electrodes under nonoscillating (p < 0.0001) relative to oscillating conditions. However, under oscillating conditions, doubling the electrode size increased MFC output significantly (p = 0.01). The stacked series MFC, with an electrode size of 10 cm2, produced the highest power density (1.2672 mW/m2) over 14 days under oscillation. Our results highlight the potentiality of H. salinarum as a viable anodic organism to produce sustained voltage in a closed-MFC system.
| S-EPMC9484973 | biostudies-literature