Project description:Brugia pahangi is a parasitic nematode that is closely related to B. malayi and Wuchereria bancrofti. B. malayi and W. bancrofti are responsible for lymphatic filariasis, affecting around 120 million people in 73 countries worldwide.This project aims to undertake high-throughput sequencing of Brugia pahangi transcriptome. The objective is to use transcriptomics to support gene finding and to recognize genes expressed in given life stages.
Project description:This study addresses the effect that the sex of the mammalian host, Meriones unguiculatus, has on the filarial nematode, Brugia pahangi
Project description:Comparative analysis of gene expression profiles provided novel insights into the genes that are transcriptionally active in infective and developing larvae of two closely related species. Species differences may indicate different metabolic adaptations that could affect host specificity, tissue tropism, and pathogenicity Two biological replicates of infective (L3) or developing larval RNA used for hybridization, in duplicate, to examine the gene expression changes in Brugia larvae Brugia malayi vector derived third stage larvae (Bm VL3); Brugia pahangi vector derived third stage larvae (Bp VL3); Brugia pahangi L3 cultured in vitro (Bp cL3); Brugia pahangi L3 derived from peritoneal cavity of infected gerbils (Bp ipL3); Brugia pahangi migrating L3 (Bp mL3) from infected gerbils
Project description:Illumina sequencing of small RNAs from Brugia pahangi and Haemonchus contortus 4 samples examined, larval stage 3 and mixed sex adults from two parasitic nematode species.