Project description:This study examined the protective effect of Chlorella vulgaris protein isolate (CPI) on the biological activity of Lacticaseibacillus rhamnosus GG (LGG) during lyophilisation, storage, and in vitro digestion. Prior to lyophilisation, probiotic hydrogels were either fermented to pH 4.5 (CF) or left untreated (CNT). The microstructure, physicochemical, and thermal properties of the resulting probiotic powders were analysed, along with LGG adhesion to an in vitro intestinal epithelium model. Embedment in CPI-based matrices provided effective lyoprotection, with CNT offering greater stability than CF. Elevated storage temperatures and relative humidity (RH) accelerated LGG inactivation, particularly in CF. While a sufficient proportion of LGG survived the harsh conditions during in vitro digestion, pre-fermentation had a negligible impact. Additionally, a satisfactory level of LGG adhesion to the intestinal mucus layer was observed. Overall, CPI exhibits strong stabilizing properties for LGG viability and represents a promising plant-based alternative to dairy proteins for probiotic food formulations.
Project description:The human microbiota is believed to influence health. Microbiome dysbiosis may be linked to neurological conditions like Alzheimer’s disease (AD), amyotrophic lateral sclerosis (ALS) and Huntington’s disease (HD). We report the ability of a probiotic bacterial strain in halting neurodegeneration phenotypes. We show that Lacticaseibacillus rhamnosus HA-114 is neuroprotective in C. elegans models of ALS and HD. Our results show that neuroprotection from L. rhamnosus HA-114 is unique from other L. rhamnosus strains, and resides in its fatty acid content. Neuroprotection by L. rhamnosus HA-114 requires acdh-1/ACADSB, kat-1/ACAT1 and elo-6/ELOVL3/6, which are key fatty acid metabolism and mitochondrial b-oxidation genes. Our data suggest that disrupted lipid metabolism contributes to neurodegeneration and that dietary intervention with L. rhamnosus HA-114 restores lipid homeostasis and energy balance through mitochondrial b-oxidation. L. rhamnosus HA-114 is suitable for human consumption opening the possibility of modifying disease progression by dietary intervention.
Project description:The presence of tagatose in Lactobacillus rhamnosus strain GG caused induction of a large number of genes associated with carbohydrate metabolism including the phosphotransferase system. In addition, these results indicate the tagatose enhanced the growth of Lactobacillus casei 01 and Lactobacillus rhamnosus strain GG and their probiotic activities by activating tagatose-associated PTS networks.
Project description:The present study reports comparative surfacomics (study of cell-surface exposed proteins) of the probiotic Lactobacillus rhamnosus strain GG and the dairy strain Lc705.