Project description:ATAC-seq profiling of Nfat5 KO and wild type macrophages derived from bone marrow (primary cells), treated or not with Lipopolysaccharide (LPS).
Project description:To gain insight into ASF1B function during erythropoiesis, RNA-seq was performed in CD71/Ter119 Fluorescence-Activated Cell Sorting (FACS) cells of WT and ASF1B KO E14.5 fetal liver(S0-S5) and bone marrow(E1-E4). Notably, depletion of ASF1B in E14.5 E4/E5 fetal liver cells significantly reduced the expression of genes associated with erythroid differentiation. In contrast, depletion of ASF1B in E4 bone marrow cells significantly reduced the expression of genes enriched in chromatin assembly.
Project description:We cultured bone marrow derived dendritic cells from WT and CD11c KO mice. Then, a group of bone marrow dendritic cells were stimulated with LPS overnight. We obtained bone marrow derived dendritic cells with or without LPS stimulation and analyzed proteomics profiles.
