Project description:To determine mRNA expression levels glp-4(bn2); let-7(wild-type) and glp-4(bn2); let-7(mn112) animals, RNA was purified from total worms collected at late L4 stage using TRI Reagent (MRC).
Project description:Transcriptome analysis of a population of control animals and RNAi-treated to partially inactivate genes that are homologs to human genes causing Retinitis Pigmentosa. RNA-seq analyses were performed at L3 larval stage in wild type, and smg-1(r861) mutants that have a defective Non-mediated decay pathway. RNA-seq experiments were also performed in adults glp-4(bn2) mutants that lack of germline.
