Project description:We have analyzed regions of accessible chromatin in adult mushroom body neurons of Drosophila melanogaster of a control genetic background using ATAC-seq.
Project description:Absolute (molar) quantification determines proteins stoichiometry in complexes, networks and metabolic pathways. We employed MS Western workflow to determine molar abundances of proteins critical for morphogenesis and phototransduction (PT) in eyes of Drosophila melanogaster using a single chimeric 264 kDa protein standard that covers, in total, 197 peptides from 43 proteins. Each protein was independently quantified with 2 to 4 proteotypic peptides with the coefficient of variation of less than 15 %, better than 1000-fold dynamic range and sub-femtomole sensitivity. We determined molar abundances and stoichiometric ratios of the components of the PT machinery and the rhabdomere, and how they are changing when rhabdomere morphogenesis is perturbed by genetic manipulation of the evolutionary conserved gene crumbs (crb).
Project description:We have analyzed transcript level changes in nuclei isolated from the adult mushroom body (MB) of Drosophila melanogaster, as well from whole-head (WH) nuclear extracts. MB and WH nuclei were isolated at five time-points across a 24-hour period.
