Anopheles
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA273873 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR1777920_1.fastq.gz | Fastqsanger.gz | |||
| SRR1777920_2.fastq.gz | Fastqsanger.gz | |||
| SRR1777930_1.fastq.gz | Fastqsanger.gz | |||
| SRR1777930_2.fastq.gz | Fastqsanger.gz | |||
| SRR1777940_1.fastq.gz | Fastqsanger.gz |
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Project description:Anopheles gambiae mosquitoes are primary human malaria vectors, but we know very little about their mechanisms of transcriptional regulation. We profiled chromatin accessibility by ATAC-seq in laboratory-reared An. gambiae mosquitoes experimentally infected with the human malaria parasite Plasmodium falciparum. By integrating ATAC-seq, RNA-seq and ChIP-seq data we showed a positive correlation between accessibility at promoters and introns, gene expression and active histone marks. By comparing expression and chromatin structure patterns in different tissues, we were able to infer cis-regulatory elements controlling tissue specific gene expression and to predict the in vivo binding sites of relevant transcription factors. The ATAC-seq assay also allowed the precise mapping of active regulatory regions, including novel transcription start sites and enhancers that annotate to mosquito immune-response genes. This study is important not only for advancing our understanding of mechanisms of transcriptional regulation in the mosquito vector of human malaria, but also the information we produced is of great potential for developing new mosquito-control and anti-malaria strategies.
2020-12-22 | GSE152924 | GEO
Project description:Anopheles gambiae mosquitoes transmit the human malaria parasite Plasmodium falciparum, which causes the majority of fatal malaria cases worldwide. The hematophagous life style defines the mosquito reproductive biology and is exploited by P. falciparum for its own sexual reproduction and transmission. The two main phases of the mosquito reproductive cycle, pre-vitellogenic (PV) and post-blood meal (PBM) shape its capacity to transmit malaria. Transition between these phases is tightly coordinated to ensure homeostasis between mosquito tissues and successful reproduction. One layer of control is provided by microRNAs, well-known regulators of blood meal digestion and egg development in mosquitoes. Here, we report a global overview of tissue-specific miRNA expression during the PV and PBM phases and identify miRNAs regulated during PV to PBM transition. The observed coordinated changes in the expression levels of a set of miRNAs in the energy-storing tissues suggest a role in the regulation of blood meal-induced metabolic changes.
2017-08-25 | GSE103034 | GEO
Project description:Plasmodium ssp. are pathogens in their vertebrate hosts and also cause deleterious effects to their insect vectors. We show here, however, that Plasmodium-infected mosquitoes more efficiently accumulate energy resources (glycogen) during oocyst development, and survive better when they are starved. Microarray analysis revealed that mosquito metabolism is altered by the presence of rapidly growing oocysts in the midgut. Plasmodium infection is associated with enhanced expression of several insulin-like peptides in mosquitoes and blocking insulin-like signaling results in diminished Plasmodium development. We conclude that Plasmodium infection dramatically changes mosquito metabolism pathways, epitomized by enhanced insulin-signaling, and thereby confer a survival advantage to the insects during periods of starvation. Manipulation of this pathway may provide new strategies to influence the ability of mosquitoes to transmit the protozoa that cause malaria.
2011-12-31 | GSE32200 | GEO
Project description:Background: The mosquito Anopheles gambiae is a major vector of human malaria. Increasing evidence indicates that blood cells (hemocytes) comprise an essential arm of the mosquito innate immune response against both bacteria and malaria parasites. To further characterize the role of hemocytes in mosquito immunity, we undertook the first genome-wide transcriptomic analyses of adult female An. gambiae hemocytes following infection by two species of bacteria and a malaria parasite. Results: We identified 4047 genes expressed in hemocytes, using An. gambiae genome-wide microarrays. While 279 transcripts were significantly enriched in hemocytes relative to whole adult female mosquitoes, 959 transcripts exhibited immune challenge-related regulation. The global transcriptomic responses of hemocytes to challenge with different species of bacteria and/or different stages of malaria parasite infection revealed discrete, minimally overlapping, pathogen-specific signatures of infection-responsive gene expression; 105 of these represented putative immunity-related genes including anti-Plasmodium factors. Of particular interest was the specific co-regulation of various members of the Imd and JNK immune signaling pathways during malaria parasite invasion of the mosquito midgut epithelium. Conclusion: Our genome-wide transcriptomic analysis of adult mosquito hemocytes reveals pathogen-specific signatures of gene regulation and identifies several novel candidate genes for future functional studies.
2011-10-31 | GSE33269 | GEO
Project description:Anopheles mosquitoes are known as dominant vectors of malaria parasites and other viral and bacterial pathogens. Fine-tune interactions between host and pathogens modulate the global distribution of mosquito-borne diseases. The way of those interactions may vary between different Anopheles species providing biological advantages for ones and limitations for others. Transmitting ability of mosquitoes can also differ between species, raising the challenging but principal questions. Deciphering the genomes has opened a new era of research and allowed to uncover in depth several molecular mechanisms involved in pathogen transmission. Nowadays there is a lack of appropriate model systems to study specific vector-pathogen interactions at molecular level. Even inbred mosquito colonies represent a heterogeneous population of cells and require special care facilities. Insect cell cultures could serve as a useful alternative due to the ease of handling and maintaining, especially for genomic studies requiring millions of cells per experiment. Here, we provide chromatin contacts heatmap and a comprehensive dataset of epigenetic characteristics, including histone marks profiles as well as RNA-seq data, for MSQ43 - established earlier cell line, initially obtained from Anopheles stephensi larva, known as a keynote malaria vector in Southeast Asia. We assume that our genomic data will be of interest for scientists who can potentially/alternatively use MSQ43 as a model system for mosquito genomic studies.
2025-09-30 | GSE278005 | GEO
Project description:Anopheles coluzzii (African malaria mosquito)
| PRJNA648422 | ENA
Project description:Anopheles mosquitoes are a potential transmitter of viruses and parasites that cause human diseases. An. albimanus is among the main malaria vectors in Mexico and Latin America. Mosquitoes' ability to transmit pathogens (vectorial competence) is directly related to their genetics and immunity. The innate immunity system is the first line of defense against non-self microbial invaders. The mosquito midgut is a crucial entry point for such microbial invaders and for mosquito immune interactions with them. Here, we provide evidence that the lola gene expression is upregulated by immune challenge in three mosquito tissues and cells: the midgut, fat body, and hemocytes. We applied an efficient method for double-stranded RNA (dsRNA)-mediated silencing of this gene expression in vivo as assessed by RT-qPCR. A transcriptome analysis was conducted to investigate the potential involvement of the Lola transcription factor in regulating gene expression. We combined RNAseq with reverse genetics, and our transcriptome analysis revealed that Lola participates in mosquito defense mechanisms. The knockdown of lola showed that it regulates the expression of genes associated with multiple functions, especially those related to detoxification and immunity. Here, we show for the first time a list of mosquito immunity genes that require Lola expression. These lola-dependent genes include antimicrobial peptides, C-lectins, pattern recognition receptors, Clip-domain serine proteases, ML-domain-containing proteins, enzymes that catalyze the generation and detoxification of reactive oxygen species, and components of the JNK immune signaling pathways in the midgut of An. albimanus. Our studies provide direct evidence of a functional link between JNK signaling and Lola factor in the mosquito midgut. Dysregulation of this pathway may contribute to increased pathogen invasion.
2026-06-01 | GSE317204 | GEO
Project description:Gene expression during the late ookinete and oocyst stages of Plasmodium falciparum (human malaria parasite) development has not been previously described in detail. We used single-cell RNA-seq to map gene expression changes in both parasite and mosquito cells and their interactions at multiple timepoints after an infectious blood meal, and in control (dsGFP-injected) and EcR-depleted (dsEcR-injected) mosquitoes.
2025-08-18 | GSE284537 | GEO
Project description:Mosquito vector of human malaria in Asia and the Middle East
| PRJNA43365 | ENA
Project description:This is a coupled multiscale mathematical model of malaria control and elimination containing four submodels: mosquito-to-human transmission of the malaria parasite, human-to-mosquito transmission of the malaria parasite, a within-mosquito malaria parasite population dynamics sub-model and a within-human malaria parasite population dynamics sub-model.
Model is encoded by Johannes and submitted to BioModels by Ahmad Zyoud.
2020-03-20 | MODEL2003190008 | BioModels