Bos indicus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA275547 | ENA |
REPOSITORIES: ENA
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Project description:Changes in gene expression induced by the Cryotop vitrification procedure in bovine blastocysts using Agilent EmbryoGENE microarray slides. Bovine in vitro-produced embryos at the blastocyst stage (144 to 156 hours post insemination) were vitrified using the Cryotop system and compared with non-vitrified (control) embryos. After vitrification, the embryos were warmed and cultured for an additional 4 hours. Embryos that re-expanded or developed to the expanded blastocyst stage were used for microarray analysis.
2015-08-03 | GSE65953 | GEO
Project description:Changes in gene expression induced by the Cryotop vitrification procedure in bovine blastocysts using Agilent EmbryoGENE microarray slides. Bovine in vitro-produced embryos at the blastocyst stage (144 to 156 hours post insemination) were vitrified using the Cryotop system and compared with non-vitrified (control) embryos. After vitrification, the embryos were warmed and cultured for an additional 4 hours. Embryos that re-expanded or developed to the expanded blastocyst stage were used for microarray analysis. Four pools of vitrified embryos were hybridized against four pools of control embryos in a dye-swap design.
2015-08-03 | E-GEOD-65953 | biostudies-arrayexpress
Project description:Transcription profile of the bovine pretransfer endometrium based on pregnancy success after in vitro produced embryos transfer
| PRJNA151847 | ENA
Project description:Affymetrix Human GeneChips are used to profile gene expression of bovine tissues and embryos to identify uniquely expressed genes in bovine in-vitro fertilized embryos by comparing with seven bovine adult tissues through gene clustering
2007-07-19 | E-GEOD-1638 | biostudies-arrayexpress
Project description:The inherently low reproductive efficiency of cattle is a major constraint on the development of the cattle industry. Embryo vitrification and warming are of great significance for the utilization of embryo resources and the improvement of the reproductive efficiency of cattle, thereby supporting the propagation of elite breeds and biological breeding. Oxidative stress is a major factor reducing the survival rate and developmental competence of vitrified-warmed embryos. In a previous study in cattle, the antioxidant α-ketoglutarate (AKG) was found to promote oocyte maturation and early embryonic development in vitro by alleviating oxidative stress. However, whether AKG can effectively alleviate cryoinjury by reducing oxidative stress, therefore improving quality and development of vitrified-warmed blastocysts, remains unclear. In the current study, in vitro produced bovine embryos were produced and AKG was supplemented to vitrification-warming solutions to explore its effects on the quality and developmental efficiency of vitrified-warmed blastocysts. The results revealed that vitrification and thawing markedly increased oxidative stress and apoptosis levels, while decreasing the survival rate, hatching rate, mitochondrial activity, and the ratio of ICM/TE cells in blastocysts. Supplementation with 25 μM AKG effectively alleviated oxidative stress, apoptosis, and increased the survival rate, hatching rate, mitochondrial activity as well as the ratio of ICM/TE. Single-cell RNA sequencing also showed that AKG treatment induced significant alterations in pathways associated with oxidative stress, apoptosis, mitochondrial function, and lipid metabolism in vitrified-warmed blastocysts. Collectively, these findings demonstrate that AKG improves the quality and developmental competence of vitrified-thawed bovine blastocysts by regulating oxidative stress. This study provides a theoretical basis for optimizing embryo vitrification-warming protocols, enhancing the utilization efficiency of vitrified embryos, and improving cattle reproductive performance.
2026-08-11 | GSE342680 | GEO
Project description:Embryo vitrification involves exposure to high concentrations of cryoprotectants and osmotic stress during cooling and warming. A multitude of these factors have the potential of affecting gene expression. In this study, in vitro produced bovine embryos at the blastocyst stage were submitted to vitrification. Four recipients each were used for transferring non-vitrified (80) and vitrified (80) embryos. Twelve non-vitrified and nine vitrified viable day-14 (D14) embryos were recovered by uterine flushing. RNA-seq analysis of whole embryo or isolated trophectoderm (TE) from vitrified and fresh recovered D14 embryos revealed a total of 927 genes changed their expression as a result of vitrification, among them 782 and 145 genes were up- and down-regulated, respectively. In TE isolates, vitrification resulted in 4,096 and 280 up- and down-regulated genes, respectively. In addition, we found 671 and 61 genes commonly up- and down-regulated in both vitrified whole embryo and TE. Commonly up-regulated pathways by vitrification included epithelial adherens junctions, sirtuin signaling, germ cell-sertoli cell junction, ATM signaling, NER, and protein ubiquitination pathways. The commonly down-regulated pathways included EIF2 signaling, oxidative phosphorylation, mitochondrial dysfunction, regulation of eIF4 and p70S6K signaling, mTOR signaling, sirtuin singling, and NER pathways. Our analysis identified specific pathways and implicated specific gene expression patterns important to cryopreservation affecting embryo developmental competence.
2021-03-01 | GSE159891 | GEO
Project description:miRNA profile of the bovine pretransfer endometrium based on pregnancy success after in vivo and in vitro produced embryos transfer
| PRJNA243061 | ENA
Project description:Gene expression profile of in vitro produced bovine blastocyst with and without Thyroid hormone supplementation
| PRJNA253832 | ENA
Project description:In vitro maturation (IVM) of the oocytes is a routine method in bovine embryo production. The competence of bovine oocytes to develop into embryo after IVM and in vitro fertilization (IVF) is lower as compared to in vivo preovulatory oocytes. Cumulus cells (CC) that enclose an oocyte are involved in the acquisition of oocyte quality during maturation. Using transcriptomic approach we compared cumulus cells gene expression during IVM with that in vivo preovulatory period.
2011-08-09 | GSE31261 | GEO
Project description:Transcription profile of the bovine pretransfer endometrium based on pregnancy success after in vivo produced embryos transfer
| PRJNA151729 | ENA