Project description:This project investigates hydraplexing, a chemical strategy that increases the detectable fragment-ion signal from labeled analytes in mass spectrometry (MS). The approach introduces several isobaric barcodes onto the same molecule, so that fragmentation yields multiple tag-derived fragments whose intensities accumulate. Using a tetra-amine scaffold derivatized with 2, 3, or 4 TMT tags, we characterized fragmentation patterns under higher-energy collisional dissociation (HCD), determined HCD energy settings that balance precursor transmission with efficient barcode release in MS2, and assessed quantitative performance across direct infusion, liquid chromatography (LC), and capillary electrophoresis (CE) workflows. We further examined performance in a complex environment by analyzing hydraplexed standards spiked into a single-cell Xenopus laevis digest. These experiments demonstrate that fragment-level signal summation can improve quantitative sensitivity in metabolomics- and proteomics-style analyses and suggest routes for extending multi-barcode architectures to broader MSⁿ applications.
2026-08-26 | PXD072028 | Pride
Project description:International Barcode of Life project (iBOL)
