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Musca domestica

ABSTRACT: Transcriptome Differences between Alternative Sex Determining Genotypes in the House Fly, Musca domestica

PROVIDER: PRJNA278841 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1919290.fastq.gz	Fastqsanger.gz
	SRR1919300.fastq.gz	Fastqsanger.gz
	SRR1919291.fastq.gz	Fastqsanger.gz
	SRR1919301.fastq.gz	Fastqsanger.gz
	SRR1919292.fastq.gz	Fastqsanger.gz

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Transcriptome Differences between Alternative Sex Determining Genotypes in the House Fly, Musca domestica

Project description:Sex determination evolves rapidly, often because of turnover of the genes at the top of the pathway. The house fly, Musca domestica, has a multifactorial sex determination system, allowing us to identify the selective forces responsible for the evolutionary turnover of sex determination in action. There is a male determining factor, M, on the Y chromosome (Y^M), which is probably the ancestral state. An M factor on the third chromosome (III^M) has reached high frequencies in multiple populations across the world, but the evolutionary forces responsible for the invasion of III^M are not resolved. To test if the III^M chromosome invaded because of sex-specific selection pressures, we used mRNA sequencing to determine if isogenic males that differ only in the presence of the Y^M or III^M chromosome have different gene expression profiles. We find that more genes are differentially expressed between Y^M and III^M males in testis than head, and that genes with male-biased expression are most likely to be differentially expressed between Y^M and III^M males. This suggests that male phenotypes, especially those related to male fertility, are more likely to be affected by the male-determining chromosome, supporting the hypothesis that sex-specific selection acts on alleles linked to the male-determining locus driving evolutionary turnover in the sex determination pathway. We additionally find that III^M males have a "masculinization" gene expression profile, suggesting that the III^M chromosome has accumulated an excess of male-beneficial alleles because of its male-limited transmission.

2015-03-20 | GSE67065 | GEO

Early developmental transcriptome of Musca domestica.

2016-08-04 | GSE85160 | GEO

Early developmental transcriptome of Musca domestica.

Project description:Using mRNA next generation sequencing, we analyze the transcriptome of Musca domestica embryos through five stages of early development: from syncytial blastoderm to dorsal closure stage. We select 100 embryos for five developmental stages: syncytial blastoderm, cellular blastoderm, gastrula, germ band extension and dorsal closure. A total of 10 samples of mRNA were obtained, represent two biological replicates for each stage.

2016-08-04 | E-GEOD-85160 | biostudies-arrayexpress

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Project description:Musca domestica Variation

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Function analysis and characterisation of a novel chitinase, MdCht9, in Musca domestica

Project description:To investigate how MdCht9 contribute to the development and digestion of Musca domestica, we silenced the chitinase gene 9 (MdCht9) by its dsRNA, while silenced the GFP as the control. Then we compared the differental expression genes between the test group and the control group.

2021-11-30 | GSE189491 | GEO

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Project description:Musca domestica transcriptomic unigenes

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Project description:Musca domestica Transcriptome sequencing

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Musca domestica

Project description:Musca domestica Organism overview

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A Whole Transcriptional Linkage Analysis of Gene Co-Regulation in Insecticide Resistant House Flies, Musca domestica

Project description:Illumina HiSeq 2000 RNASeq was conducted on the mRNA fraction of three adult female Musca domestica strains. Two strains were the insecticide susceptible strains CS and aabys, which has five distinct recessive morphological markers, one on each of the five autosomes in M. domestica. The third strain was ALHF, which is a highly insecticide resistant strain obtained in Alabama. The reference transcriptome was generated de novo using Trinity. The coding trimmed sequences from Augustus were then used as the mapping reference for the individual FASTQ files from each strain. trace reads were mapped using RSEM to generate FPKM estimates. Gene expression levels were compared to the ALHF strain using EdgeR to identify genes that were downregulated in both aabys and CS. RNA Seq profiling of the mRNA of adult Musca domestica with different autosomal contributions from insecticide resistant and susceptible strains (whole insect pooled samples)

2014-01-02 | E-GEOD-39327 | biostudies-arrayexpress

Musca domestica

Project description:Musca domestica Raw sequence reads

| PRJNA1175847 | ENA