Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Dual RNA-seq – Comparative Dual RNA-seq in pig macrophages

ABSTRACT: Dual RNA-seq – Comparative Dual RNA-seq in pig macrophages

OTHER RELATED OMICS DATASETS IN: GSE67950GSE67759GSE67951GSE67757GSE67948GSE67952GSE67758

PROVIDER: PRJNA281342 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR2980120.fastq.gz	Fastqsanger.gz
	SRR2980130.fastq.gz	Fastqsanger.gz
	SRR2980140.fastq.gz	Fastqsanger.gz
	SRR2980150.fastq.gz	Fastqsanger.gz
	SRR2980121.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 33

Similar Datasets

Dual RNA-seq – Comparative Dual RNA-seq in pig macrophages

Project description:Bacteria possess many small noncoding RNAs whose regulatory roles in pathogenesis are little understood due to a paucity of macroscopic phenotypes in standard virulence assays. Here, we use a novel Dual RNA-seq approach for a single-step simultaneous RNA profiling in both pathogen and host to reveal molecular phenotypes of sRNAs during infection with Salmonella Typhimurium. We identify a new PhoP/Q-activated small RNA which upon bacterial internalization acts to temporally control the expression of both, invasion-associated effectors and virulence genes required for intracellular survival. This riboregulatory activity is shown to adjust the human response to replicating Salmonella, and have a pervasive impact on host RNA expression both inside and outside protein-coding regions including infection-specific alterations of an array of long noncoding RNAs. Our study provides a paradigm for a comprehensive RNA-based analysis of intracellular bacterial pathogens without their physical purification from a host and a new discovery route for hidden functions of pathogen genes.

2015-12-11 | GSE67948 | GEO