Project description:We used a nonhuman primate (NHP) model of ligature-induced periodontitis to identify gingival transcriptome changes associated with aging during the phases of periodontitis lesions (initiation, progression, and resolution). Four age groups of nonhuman primate were studied: Young (<3 years of age); Adolescent (3 to 7 years), Adult (12 to 15 years), and Aged (17-23 years)
Project description:This study reports, for the first time, the genome scale methylome of nonhuman primate preimplantation development and proposes a perspective of DNA methylation dynamic equilibrium involving both DNA demethylation and de novoremethylation during primate early embryogenesis.
Project description:Obesity and type 2 diabetes (T2D) remain major global healthcare challenges and developing therapeutics necessitate using nonhuman primate models. Here, we present proteomic analyses of all the major organs of cynomolgus monkeys with spontaneous obesity or T2D in comparison to healthy controls.
Project description:We aimed to establish a translational aging model of testicular peritubular cells (TPCs), which is based on immortalized nonhuman primate (NHP) peritubular cells (iMKTPCs) from Callithrix jacchus. Cellular senescence was robustly induced within 10 days by exposure to 25 µg/ml bleomycin for 24 h.
Project description:Primordial germ cells (PGCs) are the earliest embryonic progenitors in the germline. Correct formation of PGCs is critical to reproductive health as an adult. Recent work has shown that primate PGCs can be differentiated from pluripotent stem cells; however, a bioassay that supports their identity as transplantable germ cells has not been reported. Here, we adopted a xenotransplantation assay by transplanting single-cell suspensions of human and nonhuman primate embryonic Macaca mulatta (rhesus macaque) testes containing PGCs into the seminiferous tubules of adult busulfan-treated nude mice. We discovered that both human and nonhuman primate embryonic testis are xenotransplantable, generating colonies while not generating tumors. Taken together, this work provides two critical references (molecular and functional) for defining transplantable primate PGCs. These results provide a blueprint for differentiating pluripotent stem cells to transplantable PGC-like cells in a species that is amenable to transplantation and fertility studies.
Project description:This project investigates how transient exposure to synthetic amyloid-β (Aβ) oligomers affects the cerebrospinal fluid (CSF) proteome of African green monkeys (AGMs), a nonhuman primate model with strong translational relevance for Alzheimer’s disease (AD). We administered synthetic Aβ oligomers and, after an 11-month interval, profiled paired proteomes from whole CSF and a CSF subcompartment enriched for extracellular vesicles.
Project description:Uterine stretch is thought to induce preterm labor in women with twin and higher order pregnancies, but the pathophysiology remains unclear. We investigated the pathogenesis of stretch-induced preterm birth for the first time in a pregnant nonhuman primate model.
