Project description:The purpose is to determine the role of TLR3-/- on the regulation of the host immune response during lethal SARS-CoV infection Groups of 10 week old C57BL6/NJ or TLR3-/- mice were infected with MA15 virus at a dose of 10^5 PFU or time-matched mock infected. Time points were 2, 4 and 7 days post infection. There were 4 or 5 animals/time point. Lung samples were collected for virus load, lung pathology, and transcriptional analysis. Weight loss and animal survival were also monitored.
Project description:The purpose of this experiment was to investigate the transcriptional differences between mice infected with icSARS CoV, SARS MA15 wild type or SARS BatSRBD viruses. Overview of Experiment: Groups of 20 week old C57BL6 mice were infected with icSARS CoV, Wild Type SARS MA15 or SARS BatSRBD mutant viruses. Infections were done at 10^4 PFU or 10^5 PFU or time-matched mock infected. Time points were 1, 2, 4 and 7 d.p.i. There were 3-5 animals/dose/time point. Lung samples were collected for virus load, transcriptional and proteomics analysis. Weight loss and animal survival were also monitored.
Project description:Lung samples were generated from female mice of MRL/MpJ-+/+(MpJ) and MRL/MpJ-lpr/lpr (lpr) on 3 days post infection of mouse-adapted SARS-CoV-2 or non-infected condition.
Project description:Lung samples were generated from male mice of C57BL/6J(B6), C57BL/6JHamSlc-ob/ob (ob/ob) and C57BLKS/J-db/db on 3 days post infection of mouse-adapted SARS-CoV-2
Project description:Lung samples were generated from male mice of C57BL/6JHamSlc-ob/ob (ob/ob) and C57BLKS/J-db/db on 3 days post infection of mouse-adapted SARS-CoV-2 or non-infected condition
Project description:To dissect the nature of the immune dysregulation induced by SARS-CoV-2 infection in mouse lungs using mouse-adapted strains of the virus. Comparisons were performed using bulk RNA-seq data from mouse (C57BL/6) lung homogenates taken 3 days post-infection with mouse-adapted SARS-CoV-2, both early and late (virulent) passages, or mock-infected mice.
Project description:Lean or obese C57BL/6JHamSlc-ob/ob (ob/ob) were developed continuous leptin (or vehicle) treatment for 6 weeks. Lung samples were generated from male mice of lean or obese ob/ob on 3 days post infection of mouse-adapted SARS-CoV-2.
Project description:The mechanisms by which pulmonary lesions and fibrosis are generated during SARS-CoV infection are not known. Using high-throughput mRNA profiling, we examined the transcriptional response of wild-type (WT), type I interferon receptor knockout (IFNAR1−/−), and STAT1 knockout (STAT1−/−) mice infected with a recombinant mouse-adapted SARS-CoV (rMA15) to better understand the contribution of specific gene expression changes to disease progression. Ten week old 129S6/SvEv wild-type, STAT1−/− (Taconic Farms, Germantown, NY), and IFNAR1−/− mice bred on a 129SvEv background were anesthetized with a ketamine and infected intranasally with either phosphate-buffered saline (PBS) alone (Invitrogen, Carlsbad, CA) or 1 × 10^5 PFU rMA15-PBS. Mice were euthanized and left lungs were harvested from individual mice (a total of 3 infected mice from each strain) at days 2, 5, and 9 postinfection (dpi) for microarray analyses. Lung samples were taken from mock-infected animals from each of the strains at 5 dpi.
Project description:We evaluated the susceptibility and lung responses of type 2 diabetic and lean mice following intranasal SARS-CoV-2 infection using the mouse-adapted MA10 strain
