Homo sapiens
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ABSTRACT:
PROVIDER: PRJNA283884 | ENA |
REPOSITORIES: ENA
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Project description:We exploited label-free quantitative mass spectrometry to compare primary human blood Dendritic cells (DCs) subsets protein expression to identify new markers. Subsets distinguished are: Plasmacytoid DCs (pDC) and BDCA3+ and CD1c+ myeloid DCs and CD16+ monocytes. The dendritic cells were analyzed by LC-MS/MS and processed by MaxQuant for identification and LFQ quantification.
2016-09-27 | PXD004678 | Pride
Project description:This SuperSeries is composed of the following subset Series: GSE24726: Gene expression profile of mature plasmacytoid dendritic cells (PDC) after the deletion of transcription factor E2-2 GSE24740: Binding targets of transcription factor E2-2 in human plasmacytoid dendritic cells Refer to individual Series
2010-12-21 | E-GEOD-24785 | biostudies-arrayexpress
Project description:<p>Plasmacytoid dendritic cells (pDC) are a subset of dendritic cells with unique immunophenotypic properties and functions. While their role in antiviral immunity through production of type I interferons is well-established, their contributions to anti-tumor immunity are less clear. While some evidence demonstrates that pDC in the tumor microenvironment (TME) may drive CD4+ T cell to become <a href="https://www.ncbi.nlm.nih.gov/gene/50943">Foxp3</a>+ T regulatory cells, little is understood about the relationship of pDC with cytotoxic CD8+ T cell, the key player in antitumor immune responses.</p> <p>In this study, we perform comprehensive immunophenotyping and functional analysis of pDC from the TME and draining lymph nodes of patients with head and neck squamous cell carcinoma (HNSCC) and identify a novel pDC subset characterized by expression of the TNF receptor superfamily member <a href="https://www.ncbi.nlm.nih.gov/gene/?term=7293">CD134 (OX40)</a>. We show that OX40 expression is expressed on intratumoral pDC in both humans and mice in a tumor-model specific fashion and that this subset of pDC enhances tumor associated-antigen (TAA)-specific CD8+ T cell responses. Through transcriptomic profiling of OX40-expressing pDC from the TME, we further characterize gene signatures unique to this pDC subset that support its role as an important immunostimulatory immune population in the TME.</p>
| phs001824 | dbGaP
Project description:Gene expression changes in neonatal and adult plasmacytoid dendritic cells (pDC) upon TLR7/8 stimulation
| PRJNA130425 | ENA
Project description:Plasmacytoid dendritic cells (pDCs) are critical antiviral sentinels known for rapid type I interferon (IFN-I) production. However, their proteomic profile and nutrient dependencies are not well understood. Here, we used absolute quantitative proteomics of ex vivo murine splenic pDCs to characterize their protein landscape. Cross-species comparison with human blood pDCs revealed strong conservation but some differences in metabolic machinery. The transferrin receptor, responsible for transferrin-iron uptake, is the most abundant nutrient transporter in both species with 40,000 copies per murine pDC, and is significantly enriched compared to conventional dendritic cells. Despite pDCs showing constitutive transferrin-iron uptake they have low expression of iron storage proteins and a modest repertoire of iron-dependent enzymes. Also, pDC do not have an increase in iron atoms per cell compared to cDC, which suggests that pDC export excess iron through ferroportin and argue that Tfrc may have iron-independent roles. Indeed, iron chelation did not affect pDC production of type I interferon. These data suggest that Tfrc supports noncanonical functions in pDC biology, possibly related to antigen uptake. This work provides new insights into pDC metabolism and iron biology, advancing understanding of their specialized immune roles. Plasmacytoid dendritic cells (pDCs) are specialized antiviral sentinels defined by rapid type I interferon (IFN‑I) production, yet their metabolic organization and metal‑ion handling remain poorly understood. Here, we establish a high‑confidence, absolute quantitative proteome of murine splenic pDCs directly ex vivo and compare it with conventional dendritic cell subsets and human pDCs. Cross‑species proteomic analysis reveals strong conservation of pDC identity alongside divergence in metabolic pathway usage. pDCs display exceptionally high expression of the transferrin receptor (TFRC) and robust transferrin uptake compared with other immune cells; however, quantitative ironome analysis demonstrates that total cellular iron content is equivalent across dendritic cell subsets. Instead, iron is differentially allocated, with pDCs enriched for iron-sulfur cluster assembly proteins, cDC1s for heme‑binding proteins, and cDC2s for non‑heme iron enzymes. Despite elevated transferrin uptake, pDCs do not accumulate intracellular iron, coincident with expression of the iron exporter ferroportin, suggesting active iron efflux. Functionally, acute chelation or supplementation of extracellular iron does not affect CpG‑A plus IFNα-induced IFNα or TNFα production by pDCs. Together, these data demonstrate that pDCs uncouple surface transferrin receptor abundance from intracellular iron accumulation and effector cytokine production, revealing a distinct organization of iron handling that may support specialized trafficking or sensing functions rather than metabolic iron demand.
2026-06-29 | PXD079623 | Pride
Project description:Differential Responses of Plasmacytoid Dendritic Cells to Influenza and Distinct Viral Pathogens
| PRJNA256332 | ENA
Project description:Langerhans cell histiocytosis (LCH) is a disease characterized by the accumulation of eponymous CD1a+ Langerin+ Langerhans-cell (LC)-like dendritic cells (DC) of largely unknown origin. Here we have performed comparative transcriptome analysis of highly purified CD207+/CD1a+ Langerhans cell histiocytosis (LCH) cells derived from different locations and disease courses and three major human dendritic cell lineages: epidermal Langerhans cells, myeloid dendritic cells (mDC1) and plasmacytoid dendritic cells (pDC) in order to investigate the relationship between LCH cells and naturally occurring dendritic cells. Data obtained indicate that LCH cells form a distinct DC entity. Furthermore, we have identified transcripts that are uniquely expressed by LCH cells in comparison to LC, mDC1, and pDC, and induce LCH-specific features in human DC. Primary cells were isolated from peripheral blood (mDC1 and pDC), skin (epidermal Langerhans cells) and CD207+/CD1a+ Langerhans cell histiocytosis (LCH) cells derived from different locations. RNA was isolated from these cells ex vivo.
2012-10-24 | E-GEOD-35340 | biostudies-arrayexpress
Project description:Plasmacytoid dendritic cells [pDCs] represent a rare innate immune subset uniquely endowed with the capacity to produce substantial amounts of type-I interferons [IFN-I]. This function of pDCs is critical for effective antiviral defenses and has been implicated in autoimmunity. While IFN-I and select cytokines have been recognized as pDC secreted products, a comprehensive agnostic profiling of the pDC secretome in response to a physiologic stimulus has not been reported. We applied LC-MS/MS to catalogue the repertoire of proteins secreted by pDCs in response to challenge with live influenza H1N1. Additionally, using single-cell RNA-seq [scRNA-seq], we perform multidimensional analyses of pDC transcriptional diversification following stimulation. Our data reveal an abundance of protein species released by pDCs in addition to IFN-I, and evidence highly specialized roles within the pDC population ranging from dedicated cytokine super-producers to cells with APC-like functions. Moreover, dynamic expression of transcription factors and surface markers characterize activated pDC fates.
2021-11-12 | MSV000088375 | MassIVE
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
2010-09-25 | GSE24338 | GEO
Project description:To identify microRNA changes during plasmacytoid dendritic cell (PDC) activation, we stimulated human primary PDCs with 10ug/ml R837 (Invivogen, San Diego, CA, USA) for 4 hours.
2013-03-25 | GSE21160 | GEO