Project description:Early embryonic development is highly sensitive to both developmental stage and culture environment, and in vitro fertilization (IVF) is known to induce transcriptional and epigenetic alterations that may compromise embryo quality and developmental competence. To systematically characterize stage-specific and origin-dependent transcriptional differences, we performed RNA sequencing of porcine embryos derived in vivo and by IVF at key preimplantation stages. Transcriptomes were generated from in vivo–derived blastocysts and hatched blastocysts, IVF-derived blastocysts and hatched blastocysts, as well as IVF-derived 4-cell embryos. Comparative analyses enable the dissection of transcriptional programs associated with early cleavage, blastocyst formation, hatching, and culture-induced perturbations. This dataset provides a comprehensive resource for investigating developmental stage transitions, IVF-associated transcriptional dysregulation, and molecular determinants of embryo quality during porcine preimplantation development.
