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Botryococcus braunii strain:779

ABSTRACT: Raw sequence reads of Botryococcus braunii strain 779's transcriptome

PROVIDER: PRJNA285009 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR2040571_1.fastq.gz	Fastqsanger.gz
	SRR2040571_2.fastq.gz	Fastqsanger.gz
	SRR2040567_1.fastq.gz	Fastqsanger.gz
	SRR2040567_2.fastq.gz	Fastqsanger.gz
	SRR2120180_1.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of a moderately growing subisolate Botryococcus braunii 779 (Chlorophyta) in response to nitrogen deprivation

Project description:We report the transcriptomic response of a moderately growing subisolate from the culture of Botryococcus sp. CCALA-779 upon nitrogen deprivation (ND).

2015-08-03 | GSE71296 | GEO

Transcriptomic analysis of a moderately growing subisolate Botryococcus braunii 779 (Chlorophyta) in response to nitrogen deprivation

Project description:We report the transcriptomic response of a moderately growing subisolate from the culture of Botryococcus sp. CCALA-779 upon nitrogen deprivation (ND). Examination of transcriptomes upon nitrogen deprivation (ND). B.braunii_non_redundant_transcriptome_assembly_61220.fasta: non-redundant assembly (with 61220 in number) of B.braunii 779 transcriptome in this study B.braunii_annotated_12292.fasta: investigated ESTs (with 12292 in number, which perfectly matches with our processed RNA expression profiling) in this study

2015-08-03 | E-GEOD-71296 | biostudies-arrayexpress

Botryococcus braunii

Project description:Bacterial community composition within Botryococcus braunii cultures Raw sequence reads

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Botryococcus braunii cultivar:FACHB357

Project description:Botryococcus braunii cultivar:FACHB357 Raw sequence reads

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Botryococcus braunii CCALA778

Project description:Bacterial community present with microalgae Botryococcus braunii CCALA778 Raw sequence reads

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Botryococcus braunii UMA2

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Transcriptomic analysis of race L Botryococcus braunii AC768 revealed a race L-specific protein resembling the lycopaoctaene synthase

Project description:The green algal Botryococcus braunii (Chlorophyte) is known for accumulating high levels of hydrocarbons that are a useful alternative to fossil fuels. B. braunii is categorized into three groups based on types of their accumulated hydrocarbons: alkadiene/triene in race A, botryococcenes in race B, and lycopadiene in race L. Transcriptomic studies in race A and race B have discovered tremendous information related to the genes encoding proteins involved in hydrocarbon biosynthesis. However, transcriptome of race L has not been reported. In this study, we report a transcriptome of race L B. braunii AC768 through the de novo assembly using Hiseq platform. Our analyses indicate that photosynthesis and protein biosynthesis are the most abundantly transcribed in actively growing race L B. braunii. We show that the transcriptome of race L shares similar amounts (~20%) of mutual best-hits with that of race A or race B. Sequence homologous analyses indicate that enzymes involved in squalene and phytoene biosynthesis are well separated into geranyl-diphosphate synthase, farnesyl-diphosphate synthase, geranylgeranyl-diphosphate synthase, phytoene synthase, and squalene synthase. Both B. braunii specific enzymes botryococcene synthase SSL3 and lycopaoctaene synthase LOS are found to form distinctive subgroups in the group of squalene synthase. One of the ESTs in AC768 transcriptome that falls into the subgroup with LOS and shares >88% identity with that of LOS. Together, our results show that SSL and LOS are unique to race B and race L B. braunii subspecies, respectively. We propose that phytoene synthase in race L shares higher homolog to squalene synthase than phytoene synthase in other algae.

2019-11-21 | GSE96585 | GEO

Botryococcus braunii BOT22

Project description:Botryococcus braunii BOT22 transcriptome project

| PRJDA50789 | ENA

N-Glycoproteomic Analysis of Botryococcus Braunii

Project description:We have performed an N-glycoproteomic analysis of the green microalgae Botryococcus braunii, a promising candidate for the production of biofuels, accumulating considerable amounts of hydrocarbon oils. Thereby, three different strains have been compared: Showa (Race B), AC761 (Race B) and CCALA778 (Race A) which differ in the type of produced hydrocarbons. In total, 517 unique N-glycosylated peptides have been identified analyzing intact N-glycopeptides as well as deglycosylated, 18O-labeled peptides. Intact N-glycopeptides that harbored N-acetylhexosamine (HexNAc) at the non-reducing end were identified. Surprisingly, these GnTI-dependent N-glycans were also found to be modified with (di)methylated hexose. This type of methylated GnTI-dependent N-glycans has not been described so far.

2017-08-08 | PXD005708 | Pride

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