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Zea mays

ABSTRACT: Maize death acids, 9-lipoxygenase derived cyclopente(a)nones, display activity as cytotoxic phytoalexins and transcriptional mediators

OTHER RELATED OMICS DATASETS IN: E-GEOD-68589

PROVIDER: PRJNA286115 | ENA |

REPOSITORIES: ENA

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Peroxidase B6T173 (ZmPrx35) from insect-resistant maize (Zea mays, p84C3) kernels

Project description:Peroxidase (POD) activity was found to be positively correlated with insect resistance in maize kernels. Thus, we purified active PODs from (Zea mays, p84C3) kernels and subjected to LC-MS/MS analyses. To verify the identified putative plant PODs, we produced them by recombinant expression in E. coli. Whereas B4FFK9 did not display in vitro activity, B6T173 (ZmPrx35) was active and also exhibits a heme cofactor. B6T173 accounts for about 80% of the POD activity in the kernel, according to densitometric evaluation.

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