Cryptolaemus montrouzieri
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA286509 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR2083649_1.fastq.gz | Fastqsanger.gz | |||
| SRR2083649_2.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:Transcriptome of the ladybird beetle Cryptolaemus montrouzieri fed natural and unnatural diets
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Project description:Transcriptome of ladybird beetles under different conditions
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Project description:Cryptolaemus montrouzieri Mulsant, 1853 Transcriptome or Gene expression
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Project description:BackgroundThe ladybird beetle Cryptolaemus montrouzieri Mulsant, 1853 (Coleoptera, Coccinellidae) is used worldwide as a biological control agent. It is a predator of various mealybug pests, but it also feeds on alternative prey and can be reared on artificial diets. Relatively little is known about the underlying genetic adaptations of its feeding habits.ResultsWe report the first high-quality genome sequence for C. montrouzieri. We found that the gene families encoding chemosensors and digestive and detoxifying enzymes among others were significantly expanded or contracted in C. montrouzieri in comparison to published genomes of other beetles. Comparisons of diet-specific larval development, survival and transcriptome profiling demonstrated that differentially expressed genes on unnatural diets as compared to natural prey were enriched in pathways of nutrient metabolism, indicating that the lower performance on the tested diets was caused by nutritional deficiencies. Remarkably, the C. montrouzieri genome also showed a significant expansion in an immune effector gene family. Some of the immune effector genes were dramatically downregulated when larvae were fed unnatural diets.ConclusionWe suggest that the evolution of genes related to chemosensing, digestion, and detoxification but also immunity might be associated with diet adaptation of an insect predator. These findings help explain why this predatory ladybird has become a successful biological control agent and will enable the optimization of its mass rearing and use in biological control programs.
| S-EPMC7905881 | biostudies-literature
Project description:BackgroundCryptolaemus montrouzieri (Coccinellidae) is widely utilized as biological control agents in modern agriculture. A comprehensive understanding of its food preference can help guide mass rearing and safety management during field application of pest control. Although some studies have paid attentions to the impacts of prey shift on C. montrouzieri, little is known regarding the role of post-transcriptional regulations in its acclimation to unnatural preys.ResultsWe performed a genome-wide investigation on alternative splicing dynamics in C. montrouzieri in response to the predation transition from natural prey to unnatural ones. When feeding on undesired diets, 402-764 genes were differentially alternative spliced in C. montrouzieri. It is noteworthy that the majority of these genes (> 87%) were not differentially expressed, and these differentially spliced genes regulated distinct biological processes from differentially expressed genes, such as organ development and morphogenesis, locomotory behavior, and homeostasis processes. These suggested the functionally nonredendant role of alternative splicing in modulating physiological and metabolic responses of C. montrouzieri to the shift to undesired preys. In addition, the individuals feeding on aphids were subject to a lower level of changes in splicing than other alternative diets, which might be because of the similar chemical and microbial compositions. Our study further suggested a putative coupling of alternative splicing and nonsense-mediated decay (AS-NMD), which may play an important role in fine-tuning the protein repertoire of C. montrouzieri, and promoting its acclimation to predation changes.ConclusionThese findings highlight the key role of alternative splicing in modulating the acclimation of ladybirds to prey shift and provide new genetic clues for the future application of ladybirds in biocontrol.
| S-EPMC11481726 | biostudies-literature
Project description:Biological control is the main purpose of intentionally introducing non-native invertebrate species. The evolutionary changes that occur in the populations of the introduced biological control agents may determine the agent's efficiency and the environmental safety. Here, to explore the pattern and extent of potential genomic changes in the worldwide introduced predatory ladybird beetle Cryptolaemus montrouzieri, we used a reduced-representation sequencing method to analyze the genome-wide differentiation of the samples from two native and five introduced locations. Our analyses based on a total of 53,032 single nucleotide polymorphism loci showed that beetles from the introduced locations in Asia and Europe exhibited significant reductions in genetic diversity and high differentiation compared with the samples from the native Australian range. Each introduced population belonged to a unique genetic cluster, while the beetles from two native locations were much more similar. These genomic patterns were also detected when the dataset was pruned for genomic outlier loci (52,318 SNPs remaining), suggesting that random genetic drift was the main force shaping the genetic diversity and population structure of this biological control agent. Our results provide a genome-wide characterization of polymorphisms in a biological control agent and reveal genomic differences that were influenced by the introduction history. These differences might complicate assessments of the efficiency of biological control and the invasion potential of this species but also indicate the feasibility of selective breeding.
| S-EPMC6503826 | biostudies-literature
Project description:BackgroundOrganisms may develop into multiple phenotypes under different nutritional environments by developmental plasticity, whereas the potential costs and mechanisms of such plasticity are poorly understood. Here we examined the fitness and gene expression of nutrition-induced phenotypes in the ladybeetle, Cryptolaemus montrouzieri after having experienced varying larval food regimes.ResultsWe found that C. montrouzieri adults undergoing a variable larval food regime achieved a similar developmental time, survival, body mass and egg production as those undergoing a high larval food regime. The survival, developmental time, body mass and fecundity of the adults from a restricted larval food regime were inferior to those from the high and variable larval food regimes. However, the adults from this restricted larval food regime had a higher expression level of genes encoding immune- and antioxidant-related enzymes than those from the high and variable larval food regimes when exposed to starvation and pesticide conditions in adult life.ConclusionsThese results suggest that larval food availability in C. montrouzieri not only triggers adult phenotypic differences but also affects reproduction and expression level of genes in adult life, indicating that the larval nutritional conditions can affect adult fitness and resistance to stressful conditions through developmental plasticity.
| S-EPMC4672569 | biostudies-literature
Project description:The 1KITE project: evolution of insects
| PRJNA286478 | ENA