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Rattus norvegicus

ABSTRACT: Transcriptomic analysis of the estrogenic effect of soy protein isolate in rat uterus

PROVIDER: PRJNA286867 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2061390.fastq.gz	Fastqsanger.gz
	SRR2061391.fastq.gz	Fastqsanger.gz
	SRR2061392.fastq.gz	Fastqsanger.gz
	SRR2061393.fastq.gz	Fastqsanger.gz
	SRR2061384.fastq.gz	Fastqsanger.gz

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Similar Datasets

Changes in mammary gene expression and morphology following consumption of soy protein isolate in female Sprague-Dawley rats differs from that produced by 17b-estradiol treatment

Project description:Soy foods have been suggested to have both positive health benefits and potentially adverse effects largely as a result of their content of isoflavone phytoestrogens. Since soy protein isolate (SPI) contains isoflavones, in addition to purported health benefits, safety concerns have been raised regarding the use of SPI and soy formulas, because of potential estrogenic actions during the neonatal period, including the potential for reproductive toxicity, infertility, and the possibility of increased risk for development and recurrence of estrogen sensitive cancers such as breast cancer. In the current study, we used a rat model to compare the effects of SPI with those of 17b-estradiol (E2), on global gene expression profiles and morphology in the female rat mammary gland. Rats were either fed AIN-93G diets containing casein (CAS) or SPI beginning on postnatal day (PND) 30. Rats were ovariectomized (OVX) on PND 50 and treated with E2 or vehicle for 14 days. Microarray analysis was carried out to compare the effects of SPI and E2 alone or in combination on the mammary gene expression. The data suggest a non-estrogenic effect of SPI on the rat mammary even in the absence of endogenous estrogens.

2012-05-21 | E-GEOD-38060 | biostudies-arrayexpress

Transcriptomic analysis of the estrogenic effect of soy protein isolate in rat uterus

Project description:There are concerns regarding possible reproductive toxicity from consumption of soy including an increased risk of endometriosis and endometrial cancer. We used global uterine gene expression profiles in adult ovariectomized (OVX) female rats assessed by RNAseq to examine the estrogenicity of soy protein isolate (SPI) and the potential for feeding SPI to alter estrogen signaling in the uterus. Rats were fed AIN93G diets made with casein (CAS) or SPI from postnatal day (PND) 30. Rats were OVX on PND 50 and infused with 17 beta-estradiol (E2) or vehicle. E2 increased uterine wet weight (P<0.05) and significantly altered expression of 2084 uterine genes. In contrast, SPI feeding had no effect on uterine weight and only altered expression of 177 genes. Overlap between E2 and SPI genes was limited to 69 genes (3%). GO analysis indicated significant differences in uterine biological processes affected by E2 and SPI and little evidence for recruitment of ER alpha to the promoters of ER-responsive genes after SPI feeding. The major E2 up-regulated uterine pathways were cancer pathways and extracellular organization. SPI feeding up-regulated uterine PPAR signaling and fatty acid metabolism. The combination of E2 and SPI feeding resulted in significant regulation of 715 fewer genes relative to E2 alone. In a separate experiment, the combination of E2 and SPI reversed the ability of E2 to induce uterine proliferation in response to the carcinogen dimethybenz(a)anthracene (DMBA). These data suggest SPI does not act as a weak estrogen in the uterus but appears to be a selective estrogen receptor modulator (SERM) interacting with a small sub-set of E2-regulated genes and to be anti-estrogenic in the presence of endogenous estrogens. Rat uterus mRNA of ovariectomized adult female rats subject to four different diets (Caseine, Caseine + E2, Soy and Soy+E2 ) were sequenced, in triplicate, in an Illumina GAIIx sequencer.

2015-06-13 | E-GEOD-69819 | biostudies-arrayexpress

Changes in mammary gene expression and morphology following consumption of soy protein isolate in female Sprague-Dawley rats differs from that produced by 17b-estradiol treatment

2012-05-22 | GSE38060 | GEO

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Project description:Dietary soy effects on early rat mammary gland development

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Project description:The renoprotective effects of soy protein in the aging kidney.

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Project description:Transcriptomic response in colon of rat with increased dietary protein content

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Transcriptome Profiling of Rattus norvegicus Embryonic Stem Cells by RNA-Sequencing

Project description:In order to establish a rat embryonic stem cell transcriptome, mRNA from rESC cell line DAc8, the first male germline competent rat ESC line to be described and the first to be used to generate a knockout rat model was characterized using RNA sequencing (RNA-seq) analysis.

2016-09-02 | GSE44150 | GEO

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Project description:Expression data from uterus of SD rat during pre-implantation period.

| PRJNA247809 | ENA

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Project description:The estrogenic activity of triclosan

| PRJNA351789 | ENA

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Project description:Different effects of 17 beta estrodial and soy protein isolate on bone gene expression in the pre-pubertal female rats

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