ABSTRACT: Gene expression data of mRNA from skeletal muscle sample of pig breed Duroc and Pietrain with malignant hyperthermia syndrome homozygous negative
Project description:Gene expression data of micro RNA from skeletal muscle sample of pig breed Duroc and Pietrain with malignant hyperthermia syndrome homozygous negative
Project description:DNA methylation is an epigenetic mechanism that plays roles in gene regulation and expression. This study aims to profile DNA methylation at genome-wide scale and single base resolution using reduced representation bisulfite sequencing (RRBS) of the muscle of pigs differing in muscle metabolism and genetic background. Pietrain homozygous- positive (PiPP) and negative (PiNN) for the ryanodine receptor mutation and malignant hyperthermia-susceptible (MHS), Duroc and F2 crossbred Duroc-Pietrain homozygous-negative (DuPi) pigs were investigated to identify differentially methylated CpG sites/ regions and to integrate the results with gene expression data.
Project description:To obtain an overview of the transcriptome landscape in developing pig skeletal muscle, 81 high-quality transcriptome libraries that covered 27 developmental stages (3 biological replicates per stage) in pig skeletal muscle were produced by strand-specific rRNA-depleted total RNA sequencing (RNA-seq). We generated 8.59 billion paired-end reads (150 bp × 2) covering 1.24 Tb of sequence for RNA-seq.
Project description:Expression profiles of porcine muscle Semimembranosus were studied to identify genes beeing significantly affected by pre-slaughter stress or no stress treatment in pigs of different breed (Italian Duroc, Italian Large White or Pietrain) and differnt Halothane genotype (RYR1 locus CC-NN, CT-Nn or TT-nn) in Pietrain pigs.
Project description:Investigation of the transcriptome profile of lung dendritic cells (DCs) of two genetically different pig breeds (Pietrain and Duroc) after PRRSV infection in vitro and determination of the temporal changes in transcriptional profiles.
