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MCPyV ST activates Max target genes by recruiting TRRAP/EP400 complex

ABSTRACT: MCPyV ST activates Max target genes by recruiting TRRAP/EP400 complex

PROVIDER: PRJNA287013 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2062580.fastq.gz	Fastqsanger.gz
	SRR2062640.fastq.gz	Fastqsanger.gz
	SRR2062581.fastq.gz	Fastqsanger.gz
	SRR2062641.fastq.gz	Fastqsanger.gz
	SRR2062582.fastq.gz	Fastqsanger.gz

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MCPyV ST, MAX and the TRRAP complex cooperate to bind to Transcription Start Sites

Project description:To determine if MCPyV ST was recruited to chromatin together with MAX and the TRRAP complex, we performed chromatin immunoprecipitation (ChIP) using the validated antibodies to MCPyV ST produced in our lab, HA tagged ST, MAX and EP400 followed by next generation sequencing. De novo DNA motif analysis revealed that the canonical E-box MYC target sequence was the most frequently observed motif. Metagene analysis revealed that antibodies to MAX, EP400, ST (Ab5) and HA tagged ST showed strong enrichment in transcription start site (TSS). H3K4me3 ChIP-seq confirmed that the peaks enriched with antibodies to MAX, EP400 and ST all centered on the H3K4me3 peaks with a high degree of overlap.

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MCPyV ST activates Max target genes by recruiting TRRAP/EP400 complex

Project description:This SuperSeries is composed of the SubSeries listed below.

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EP400 is required for Max and MCPyV mediated gene activation

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EP400 Deposits H3.3 into Promoters and Enhancers During Gene Activation

Project description:Purpose: We wanted to know how histone variants H3.3 and H2AZ are deposited into genes and enhancers during gene activation Methods: U2OS cells were harvested at 70% confluency with formaldehyde crosslinking for ChIP-seq without crosslinking for RNA-seq. ChIP DNA were purified through standard chromatin immunoprecipitation using an Pol II, MED26, EP400, H3.3, H2AZ, H3k4me1 and K3K18ac antibodies. Libraries were prepared with a KAPA LTP kit and sequenced using the Illumina HiSeq 2000 platform. Total RNA was extracted with Trizol, digested with DNaseI and further purified by acid phenol. Libraries were prepared with Illumina TruSeq RNA Sample Prep Kits v2 and were sequenced on Illumina HiSeq 2000. Conclusion: Our biochemical and genomics (ChIP-Seq and mRNA-Seq) data show that EP400 contributes to H3.3 deposition in significantly with less of an effect on H2AZ in both genes and enhancers Enrichment of EP400, Pol II, MED26, Histone varinat H3.3 and H2AZ, and enhancers marks H3K4me1 and H3K18ac in either Mock and/or EP400 knockdown conditions on chromatin were generated by ChIP-Seq. mRNA profiles under Mock siRNA or EP400siRNA were generated by deep sequencing, using Illumina HiSeq 2000.

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