Project description:Mosquitos were fed sugar water or blood. The blood fed mosquitos were given 6 h or 24 h to digest. The midguts were dissected and prepared for MSP-MS.
Project description:We reported the RNA-seq based analyses of the transcriptional changes in the Aedes aegypti midguts knock down 3, 6days, feed antibody 18h transcriptome. Comparison of the midguts transcriptome of Aedes aegypti females at two knockdown time points and one feed condition; GFP dsRNA-3 or -6days: 3 or 6 days after 7day-old mosquitos were microinjected GFP dsRNA AaMesh dsRNA-3 or -6days: 3 or 6 days after 7day-old mosquitos were microinjected AaMesh dsRNA Pre-immune-18h: 18hrs after 7day-old wild type mosquitos were fed with Pre-immune AaMesh antibody-18h: 18hrs after 7day-old wild type mosquitos were fed with AaMesh antibody
Project description:Mosquito saliva contains bioactive factors that enhance viral infection, with sialokinin identified as a key contributor to vascular leakage and viral spread in mice. Understanding effect of sialokinin on human moncoytes, the most important innate cells that responsible for the viral-induced inflammatory, will provide important insight to host-virus-vector interaction in arbovirus infection.
