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Rattus norvegicus

ABSTRACT: Genome-wide DNA methylation profiling reveals epigenetic changes in the rat nucleus accumbens associated with cross-generational effects of adolescent THC exposure

PROVIDER: PRJNA287327 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2069440.fastq.gz	Fastqsanger.gz
	SRR2069450.fastq.gz	Fastqsanger.gz
	SRR2069441.fastq.gz	Fastqsanger.gz
	SRR2069451.fastq.gz	Fastqsanger.gz
	SRR2069461.fastq.gz	Fastqsanger.gz

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Genome-wide DNA methylation profiling reveals epigenetic changes in the rat nucleus accumbens associated with cross-generational effects of adolescent THC exposure

Project description:Drug exposure during critical periods of development is known to have lasting effects, increasing one’s risk for developing mental health disorders. Emerging evidence has also indicated the possibility for drug exposure to even impact subsequent generations. Our previous work demonstrated that adolescent exposure to Δ9-tetrahydrocannabinol (THC), the main psychoactive component of marijuana (Cannabis sativa), in a Long-Evans rat model affects reward-related behavior and gene regulation in the subsequent (F1) generation unexposed to the drug. Questions, however, remained regarding potential epigenetic consequences. In the current study, using the same rat model, we employed Enhanced Reduced Representation Bisulfite Sequencing to interrogate the epigenome of the nucleus accumbens, a key brain area involved in reward processing. This analysis compared 16 animals with parental-THC exposure and 16 without to characterize relevant systems-level changes in DNA methylation. We identified 1,027 differentially methylated regions (DMRs) associated with parental THC exposure in F1 adults, each represented by multiple CpGs. These DMRs fell predominantly within introns, exons, and intergenic intervals, while showing a significant depletion in gene promoters. From these, we identified a network of DMR-associated genes involved in glutamatergic synaptic regulation, which also exhibited altered mRNA expression in the nucleus accumbens. These data provide novel insight into drug-related cross-generational epigenetic effects, and serve as a useful resource for investigators to explore novel neurobiological systems underlying drug abuse vulnerability. Study consisted of a total of 32 F1 individuals (Long-Evan rats); 16 animals (8 female, 8 male) were offspring from THC-exposed parents, and the remaining 16 animals (8 female, 8 male) were offspring from saline-exposed parents ("controls"; "VEH-exposed").

2015-06-19 | E-GEOD-69984 | biostudies-arrayexpress

Genome-wide DNA methylation profiling reveals epigenetic changes in the rat nucleus accumbens associated with cross-generational effects of adolescent THC exposure

2015-06-19 | GSE69984 | GEO

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Project description:DNA methylation changes in rat sperm following exposure to THC

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Project description:Rat DNA methylation in the Nucleus Accumbens in Incubation of Cocaine Craving

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Project description:Rat gene expression in the Nucleus Accumbens in Incubation of Cocaine Craving

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Project description:The effect of alcohol on the expression of miroRNAs in rat nucleus accumbens

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Project description:Genome-wide analysis of nucleus accumbens gene expression after EtOH consumption in brown rat

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Gene expression in the nucleus accumbens shell of adult rats of both sexes after chronic exposure to delta9-tetrahydrocannabinol during adolescence

Project description:Canabinoid exposure during adolescence alters the rewarding properties of drugs of abuse by mechanisms that are not fully understood. The nucleus accumbens is a key node of the circuit mediating the rewarding effects of most drugs. Here, we present data on the differential gene expression in the nucleus accumbens shell in male and female Wistar rats (postnatal day 90) after exposure to THC (3 mg/kg i.p.) or vehicle (ethanol:cremophor:saline; 1:1:18; 2 mL/kg i.p.) during adolescence (postnatal day 28 to postnatal day 44; injections on alternate days). There were 96 DEGs in THC-males compared to VEH-males and 87 DEGs in the females’ comparison. Only nine of these DEGs were present in both the THC vs. VEH comparison in both sexes. Our results suggest that THC exposure during adolescence affects several families of genes that have important functions for brain development, behavior and synaptic plasticity and could explain, at least in part, the complex behavioral phenotype observed in adult individuals with exposure to cannabinoids during adolescence.

2020-09-19 | GSE158188 | GEO

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Project description:Gene expression data from brain nucleus accumbens shell (Acb-sh) following binge-like alcohol drinking by adolescent alcohol-preferring rats

| PRJNA212739 | ENA

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Project description:Genome-wide analysis of nucleus accumbens gene expression after ethanol consumption in rat

| PRJNA293265 | ENA