Ontology highlight
ABSTRACT:
PROVIDER: PRJNA287549 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The objective of this study was to determine if Salmonella colonization of chickens could be reduced through competitive exclusion using a defined community of chicken commensal bacteria. One-day old White Leghorn chicks, hatched on-site, were randomly divided into experimental groups and given an oral gavage of either a defined community of 15 bacterial species (DC), cecal contents (CC), or sterile PBS (control; CT). After one week, birds were euthanized for cecal content collection (pre-Salmonella sample) while the remaining birds were orally gavaged 1 X 10^8 colony forming units (CFU) of Salmonella enterica ser. Heidelberg strain 2813 (SH2813). Bacterial counts for three post-Salmonella timepoints (3, 14, and 28 days post inoculation; dpi) were evaluated. Bacteriological enumeration was performed by plating cecal contents onto Salmonella selective agar to determine CFU/g in each group for all collection days. Cecal contents were also used for 16S amplicon sequencing. Cecal tissue was used for stranded mRNA sequencing (RNA-Seq).
2026-01-30 | GSE286977 | GEO
Project description:RNA was isolated from laser capture micro-dissected (LCM) tumour nests from fresh frozen skin of K14Cre-ER; Ptch1fl/fl; p53fl/fl mice either before (untreated) or after (treated) 28 days of twice a day vismodegib dosing at 75mg/kg body weight by oral gavage. The "SAMPLE_ID" sample characteristic is a sample identifier internal to Genentech. The ID of this project in Genentech's ExpressionPlot database is PRJ0014355
2018-08-08 | GSE101504 | GEO
Project description:We previously reported that skeletal muscle adaptation to regular exercise requires a healthy gut microbiome, contributing to growing evidence that some exercise benefits are mediated by microbiome-derived metabolites. Here, to identify such exercise-associated microbial metabolites, we transfer cecal contents from exercise-trained donor mice into exercise-naïve recipient mice undergoing unilateral hindlimb immobilization. Recipients of cecal material from exercise-trained donors exhibit less muscle atrophy compared with those receiving transfers from sedentary donors. Untargeted metabolomics reveal metabolites enriched in cecal content, serum, and muscle of recipients from exercise-trained donors, consistent with microbial origin. Oral administration of two such metabolites (pipecolic acid and succinate) attenuates muscle atrophy and preserves muscle function in exercise-naïve mice, potentially by enhancing cellular energy status and translational capacity. These findings further define the gut microbiome-skeletal muscle axis and provide evidence that exercise-associated microbial metabolites serve as a novel class of exercise mimetics for treating conditions responsive to physical activity.
2026-05-27 | PXD077599 | Pride
Project description:Type 2 Diabetes Mellitus (T2DM) is associated with a state of chronic low-grade inflammation that severely affects the central nervous system, particularly the hypothalamus, which acts as the master regulator of energy homeostasis. This study aimed to characterize the hypothalamic transcriptomic signature in a complex murine model of T2DM induced by neonatal administration of Streptozotocin (nSTZ, 70 mg/kg) followed by a chronic High-Fat Diet (HFD, 60% kcal from fat). Given the neuroprotective properties of natural polyphenols, we evaluated the modulatory effects of Curcumin (50 mg/kg) and Aged Garlic Extract (EAE, 200 mg/kg) administered daily via oral gavage for 28 days. By employing high-throughput RNA sequencing (RNA-seq), we identified differentially expressed genes (DEGs) and perturbed molecular pathways involved in neuroinflammation, synaptic plasticity and gabaergic system. Our findings provide a comprehensive map of the gene expression changes associated with diabetic neurodegeneration and the therapeutic potential of nutraceutical interventions to restore hypothalamic function.
2026-05-04 | GSE329555 | GEO
Project description:Mice were treated with either 100mg/kg baclofen or 0.5% methylcellulose alone by oral gavage for 1 or 5 days.
2010-01-12 | GSE17266 | GEO
Project description:Interventions: A group: Oral administration of Daikenchuto (15g/day t.i.d. before meals) for 28 days. Thereafter non-treatment for 28 days.
B group: Non-treatment for 28 days. Thereafter oral administration of Daikenchuto (15g/day t.i.d. before meals) for 28 days.
Primary outcome(s): 1)total number of elimination of the radiopaque markers 2) oro-cecal transit time 3) GSRS (Japanese Version) Score 4) VAS 5) GVS
Study Design: Cross-over Randomized
| 2622806 | ecrin-mdr-crc