Project description:Proteomic profiling of insoluble aggregates in Lacticaseibacillus paracasei L9 via DIA-MS. To investigate how Hsp20-mediated protein aggregation promotes dormancy, we isolated insoluble proteins from eight samples: non-adapted cells, acid-adapted cells, the hsp20-overexpression strain, the empty vector control, and each of these after lethal acid challenge
Project description:Purpose: To understand the bile salts resistance mechanisms in L. paracasei L9 Methods: Samples from L9 cultured with or without bile salts were sequenced on an Illumina Hiseq platform. Three independent biological replicates were produced including 6 samples in total. Results: Raw data were firstly processed through in-house perl scripts to generate clean data, and then clean date were mapped to the reference genome, getting about 8-10 million total mapped reads per sample.