Project description:To explore the molecular events in LMH cells upon infectious laryngotracheitis virus (ILTV) infection, transcriptional profiling of LMH cells infected with ILTV was compared with uninfected control cells.
Project description:To explore the molecular events in LMH cells upon infectious laryngotracheitis virus (ILTV) infection, transcriptional profiling of LMH cells infected with ILTV was compared with uninfected control cells. Gene expression in LMH cells with or without virus infection was measured at two day post infection. Four independent experiments were performed.
Project description:Transcriptional profiling of chicken embryo lung cells infected infectious laryngotracheitis virus (ILTV) comparing control uninfected lung cells. Goal was to determine the changes of host gene expression by ILTV infection and host-virus interaction.
Project description:Host gene expression of chicken embryo lung cells infected an infectious laryngotracheitis virus (ILTV) vaccine strain comparing control uninfected lung cells. Goal was to identify the changes of host gene expression by ILTV vaccine infection.
Project description:Transcriptional profiling of chicken embryo lung cells infected infectious laryngotracheitis virus (ILTV) comparing control uninfected lung cells. Goal was to determine the changes of host gene expression by ILTV infection and host-virus interaction. Two-condition experiment, uninfected chicken embryo lung cells vs. infected chicken embryo lung cells. Biological replicates: 1 control uninfected sample, 1 infected experimental sample at each 1dpi, 3dpi, 5dpi, and 7 dpi.
Project description:To gain the in-depth insights into the transcriptional regulation network of chicken liver cancer cells, we sequenced the mRNA of LMH cells for further genome-wide gene expression analysis. To investigate the transcriptional regulation network of chicken p53, we created conditions different in p53 activity for LMH cell lines upon ILTV infection. We then performed gene expression profiling analysis using data obtained from RNA-seq of LMH cells.
Project description:Infectious laryngotracheitis (ILT) is an acute, contagious, upper respiratory disease, which is caused by gallid herpesvirus 1 (GaHV-1). Due to the mortality rates up to 70% depending on the virulence of the virus, it is of economic importance of the disease to explore the etiology of the ILT in the poultry industry. In this study, 15-day-old SPF white leghorn chickens were used to transcriptome analysis in chicken trachea immunized with infectious laryngotracheitis virus vaccine. In conclusion, chicken embryo origin (CEO) vaccine activation of the MHC-I and MHC-II pathways provides insight into the molecular mechanism of immune response in chickens, and holds potential for evaluation and design of new ILT vaccines in a manner adapted to the host immune response to the virus.
