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Homo sapiens

ABSTRACT: Small RNA sequencing of human K562 cells transduced with lentiviral vectors overexpressing RUNX1

PROVIDER: PRJNA289985 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2103380.fastq.gz	Fastqsanger.gz
	SRR2103381.fastq.gz	Fastqsanger.gz
	SRR2103382.fastq.gz	Fastqsanger.gz
	SRR2103383.fastq.gz	Fastqsanger.gz
	SRR2103384.fastq.gz	Fastqsanger.gz

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Small RNA sequencing of human K562 cells transduced with lentiviral vectors overexpressing RUNX1

Project description:miRNA-Sequencing of RUNX1 overexpressing K562 cells were compared to K562 cells transduced with an empty vector control to analyse the differential expression of miRNAs in hematopoiesis. K562 cells were transduced either with an empty lentiviral vector as control or a lentiviral vector overexpressing human RUNX1. The samples were biological triplicates and the results were validated by qRT-PCR using SYBR green.

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Small RNA sequencing of human K562 cells transduced with lentiviral vectors overexpressing RUNX1

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Project description:The 22Rv1 and PC-3 cells were transduced with shMIMIC human lentiviral vectors (Horizon Discovery, Cambridge, UK) to stably overexpress miRNA-23c or -4328. The SMARTvector Non-Targeting Control (NTC) was expressed to serve as a negative control. The vectors contained a turbo green fluorescent protein (turboGFP) and a puromycin resistance gene cassette. After transduction, cells were cultured in a medium supplemented with 5 µg/mL puromycin (Takara Bio, Tokyo, Japan) for antibiotic selection. Overexpression was confirmed by monitoring the turboGFP by fluorescence microscopy and by RT-qPCR analysis of miRNA-23c and -4328 levels. Relative protein quantification was performed to compare protein expression in 22Rv1 and PC-3 single cell clones overexpressing miRNA-23c and -4328, compared to corresponding NTC cells. Single cell clones overexpressing either miRNA-23c (n = 3), -4328 (n = 3) or NTC (n = 3) were analyzed in triplicate.

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