Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mus musculus

ABSTRACT: H3.3 deposition at endogenous retroviruses in embryonic stem cells

PROVIDER: PRJNA290025 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR2103630.fastq.gz	Fastqsanger.gz
	SRR2103621.fastq.gz	Fastqsanger.gz
	SRR2103631.fastq.gz	Fastqsanger.gz
	SRR2103622.fastq.gz	Fastqsanger.gz
	SRR2103632.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

H3.3 deposition at endogenous retroviruses in embryonic stem cells

Project description:We report DAXX-dependent deposition of histone H3.3 at at endogenous retroviruses in embryonic stem cells.

2015-07-16 | GSE70966 | GEO

H3.3 deposition at endogenous retroviruses and ERV reactivation in Daxx knock-out embryonic stem cells

Project description:H3.3 deposition at endogenous retroviruses and ERV reactivation in Daxx knock-out embryonic stem cells

| PRJNA290023 | ENA

Mus musculus

Project description:PARP1 inhibition activates endogenous retroviruses and cell death in embryonic stem cells

| PRJNA743896 | ENA

Histone H3.3 lysine 9 and 27 control repressive chromatin states at cryptic cis-regulatory elements and bivalent promoters in mouse embryonic stem cells

Project description:Histone modifications are associated with distinct transcriptional states, but it is unclear whether they instruct gene expression. To investigate this, we mutated histone H3.3 K9 and K27 residues in mouse embryonic stem cells (mESCs). Here, we find that H3.3K9 is essential for controlling specific distal intergenic regions and for proper H3K27me3 deposition at promoters. The H3.3K9A mutation resulted in decreased H3K9me3 at regions encompassing endogenous retroviruses and induced a gain of H3K27ac and nascent transcription. These changes in the chromatin environment unleashed cryptic enhancers, resulting in the activation of distinctive transcriptional programs and culminating in protein expression normally restricted to specialized immune cell types. The H3.3K27A mutant disrupted deposition and spreading of the repressive H3K27me3 mark, particularly impacting bivalent genes with higher basal level of H3.3 at promoters. Therefore, H3.3K9 and K27 crucially orchestrate repressive chromatin states at cis-regulatory elements and bivalent promoters, respectively, and instruct proper transcription in mESCs.

2024-06-24 | MSV000095134 | MassIVE

Morc3 silences endogenous retroviruses in mouse embryonic stem cells

Project description:Morc3 silences endogenous retroviruses in mouse embryonic stem cells

| PRJNA727542 | ENA

Mus musculus

Project description:TRIM28 silences endogenous retroviruses in neural progenitor cells

| PRJNA196678 | ENA

Mus musculus

Project description:Genome-wide analysis of H3.3 dissociation in mouse embryonic stem cells

| PRJNA268514 | ENA

Morc3 silences endogenous retroviruses in mouse embryonic stem cells [Morc3_mESCs_RNAseq]

Project description:Morc3 silences endogenous retroviruses in mouse embryonic stem cells [Morc3_mESCs_RNAseq]

| PRJNA727549 | ENA

Mus musculus

Project description:A hyperdynamic H3.3 nucleosome marks promoter regions in pluripotent embryonic stem cells

| PRJNA348448 | ENA

Histone H3.3 is required for endogenous retroviral element silencing and genome stability [RNA-Seq]

Project description:Endogenous retroviruses (ERVs) have provided an evolutionary advantage in the diversification of transcript regulation and are thought to be involved in the establishment of extraembryonic tissues during development. However, silencing of these elements remains critical for the maintenance of genome stability. Here, we define a new chromatin state that is uniquely characterized by the combination of the histone variant H3.3 and H3K9me3, two chromatin ‘marks’ that have previously been considered to belong to fundamentally opposing chromatin states. H3.3/H3K9me3 heterochromatin is fundamentally distinct from ‘canonical’ H3K9me3 heterochromatin that has been under study for decades and this unique functional interplay of a histone variant and a repressive histone mark is crucial for silencing ERVs in ESCs. Our study solidifies the emerging notion that H3.3 is not a histone variant associated exclusively with “active” chromatin and further suggests that its incorporation at unique heterochromatic regions may be central to its function during development and the maintenance of genome stability. RNA-seq analysis of three embryonic stem cell lines WT, H3.3 KO1, and H3.3 KO2)

2015-05-05 | E-GEOD-59104 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data