Project description:We sequenced Endogenous short RNAs in Mucor circinelloides fungus grown in standard liquid culture. Short RNAs were profiled in wild type, Dicer-like 1 mutant (dcl1-), Dicer-like 2 mutant (dcl2-) and double Dicer mutant (dcl1-/dcl2-) strains. We identified many loci that produced less short RNAs in the dcl2- strain suggesting that DCL2 is the major protein generating short RNAs in Mucor circinelloides.
Project description:ChIP-seq data for three Mucor spp. from the Mucor circinelloides species complex: PS15, PS14, and PS10. ChIP was performed against H3K9me2 and RNA polymerase II (RNAP). PS10 and PS14 include samples from a wild type (WT) and an epimutant strain (E1 and E3, respectively), while PS15 include a WT from each mating type (- and +), two epimutant strains (E1m, E10p) and their progeny (#10 and #34, respectively)
Project description:Transcriptional profiling of wild-type strain (R7B) of the fungus Mucor circinelloides comparing control mycelia grown for 18 hours in the dark with mycelia grown in the same conditions and illuminated with white light for 20 min. Goal was to identify genes regulated by a pulse of white light.
Project description:We sequenced Endogenous short RNAs in Mucor circinelloides fungus grown in standard liquid culture. Short RNAs were profiled in wild type, Dicer-like 1 mutant (dcl1-), Dicer-like 2 mutant (dcl2-) and double Dicer mutant (dcl1-/dcl2-) strains. We identified many loci that produced less short RNAs in the dcl2- strain suggesting that DCL2 is the major protein generating short RNAs in Mucor circinelloides. Endogenous short RNAs were profiled in wild type, single and double dcl mutant strains.
Project description:The filamentous fungus Mucor circinelloides FRR5020 (Cir1 in sample name) was grown to investigate the transcriptomic response to calcium availability under reference phosphate conditions. Cultures were grown in nitrogen-limited medium containing inorganic phosphate at the reference concentration P1 (7 g/L KH₂PO₄ and 2 g/L Na₂HPO₄), with two calcium treatments: calcium present at 0.1 g/L CaCl₂·2H₂O (Ca1) and calcium missing (Ca0). Biomass was harvested after 5 days of cultivation (4 biological replicates per treatment), RNA was extracted, and transcriptomes were sequenced for differential expression analysis between the calcium treatments.
Project description:The filamentous fungus Mucor circinelloides VI04473 (Cir2 in sample name) was grown to investigate the transcriptomic response to calcium availability under reference phosphate conditions. Cultures were grown in nitrogen-limited medium containing inorganic phosphate at the reference concentration P1 (7 g/L KH₂PO₄ and 2 g/L Na₂HPO₄), with two calcium treatments: calcium present at 0.1 g/L CaCl₂·2H₂O (Ca1) and calcium missing (Ca0). Biomass was harvested after 5 days of cultivation (4 biological replicates per treatment), RNA was extracted, and transcriptomes were sequenced for differential expression analysis between the calcium treatments.
Project description:Here, we have studied the first stage of this infection—the interaction of Mucor circinelloides spores with phagocytic cells—from an integrated transcriptomic and functional perspective. Our transcriptomic results showed that a relevant gene network is remodelled in response to phagocytosis, enriched in crucial functions to survive the phagosome, such as nutritional adaptation and response to oxidative stress. An additional transcriptomic analysis of atf1 and atf2 mutants unveiled the complex gene network of secondarily regulated genes involved in the response to phagocytosis. These new insights into the initial phase of mucormycosis define genetic regulators and molecular processes that could serve as pharmacological targets.
